Immunocytochemical demonstration of a new vimentin-associated protein in 3T3 fibroblasts

Seu M. Wang, Jin Shan Chen, Tsorng Harn Fong, Jiahn Chun Wu

Research output: Contribution to journalArticle

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Abstract

Using a xanthophore cytoskeletal preparation as immunogen, we have produced a monoclonal antibody, A2, which recognized a 160 kDa protein in 3T3 fibroblasts. This protein makes up a cytoplasmic filamentous system, which colocalizes with vimentin filaments. When microtubules and actin filaments are dissolved by high salt extraction, staining with antibody A2 is unaffected. Immunoblot analysis confirms that the 160 kDa protein is co-isolated with vimentin during in vivo high salt extraction. Following vinblastine treatment, both the 160 kDa protein and vimentin become localized to perinuclear caps, as do other intermediate filaments and their associated proteins; after vinblastine removal, the immunostaining produced by A2 becomes filamentous. Immunoelectron microscopy demonstrates that antibody A2 stains a filament system with a diameter of about 10 nm. Our observations suggest that the 160 kDa protein may be a new vimentin-associated protein which differs from the intermediate filament-associated proteins previously reported, and is widely distributed in several cell types.

Original languageEnglish
Pages (from-to)469-476
Number of pages8
JournalHistochemical Journal
Volume28
Issue number7
Publication statusPublished - 1996
Externally publishedYes

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Vimentin
Fibroblasts
varespladib methyl
Proteins
Vinblastine
Salts
Intermediate Filament Proteins
Intermediate Filaments
Immunoelectron Microscopy
Antibodies
Actin Cytoskeleton
Microtubules
Coloring Agents
Monoclonal Antibodies
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Immunocytochemical demonstration of a new vimentin-associated protein in 3T3 fibroblasts. / Wang, Seu M.; Chen, Jin Shan; Fong, Tsorng Harn; Wu, Jiahn Chun.

In: Histochemical Journal, Vol. 28, No. 7, 1996, p. 469-476.

Research output: Contribution to journalArticle

Wang, Seu M. ; Chen, Jin Shan ; Fong, Tsorng Harn ; Wu, Jiahn Chun. / Immunocytochemical demonstration of a new vimentin-associated protein in 3T3 fibroblasts. In: Histochemical Journal. 1996 ; Vol. 28, No. 7. pp. 469-476.
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