Immune-regulatory activity of methanolic extract of Acacia confusa heartwood and melanoxetin isolated from the extract

Shang Tse Ho, Yu Tang Tung, Yu Jung Wu, Chi Chen Lin, Jyh Horng Wu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The antioxidant, anti-inflammatory, antivirus, uric acid decreasing, and hepatoprotective activities of Acacia confusa extracts were demonstrated in previous studies. However, there is no scientific evidence concerning the immune-regulatory activity of the heartwood extract of A. confusa. In this study, the effect of a methanolic heartwood extract (MHE) from A. confusa on dendritic cell (DC) activation and function was examined. A. confusa MHE significantly reduced the production of pro-inflammatory cytokine interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated DCs, and the effective concentration (25 μg ml-1) of A. confusa MHE did not affect cell viability. Additionally, the bioactive phytochemical from A. confusa MHE, melanoxetin, was isolated and purified by HPLC. This substance inhibited the production of pro-inflammatory cytokines (tumor necrosis factor-α, IL-6, and IL-12) in LPS-stimulated DC at a concentration of 12.5 μM. Moreover, the expression levels of co-stimulatory molecules such as CD40, CD80, and CD86 also remarkably decreased after treatment with melanoxetin at the same dose. These findings indicate that A. confusa MHE and melanoxetin have excellent immune-suppressive activity and may be potential candidates for further development of natural health supplements.

Original languageEnglish
Pages (from-to)645-652
Number of pages8
JournalHolzforschung
Volume69
Issue number5
DOIs
Publication statusPublished - Jul 1 2015
Externally publishedYes

Fingerprint

Lipopolysaccharides
Interleukin-6
Cytokines
Phytochemicals
Interleukin-12
Uric Acid
Antioxidants
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Chemical activation
Cells
Health
Molecules
Acids
Dendritic Cells

Keywords

  • Acacia confusa
  • dendritic cell
  • heartwood
  • immune-regulatory activity
  • melanoxetin

ASJC Scopus subject areas

  • Biomaterials

Cite this

Immune-regulatory activity of methanolic extract of Acacia confusa heartwood and melanoxetin isolated from the extract. / Ho, Shang Tse; Tung, Yu Tang; Wu, Yu Jung; Lin, Chi Chen; Wu, Jyh Horng.

In: Holzforschung, Vol. 69, No. 5, 01.07.2015, p. 645-652.

Research output: Contribution to journalArticle

Ho, Shang Tse ; Tung, Yu Tang ; Wu, Yu Jung ; Lin, Chi Chen ; Wu, Jyh Horng. / Immune-regulatory activity of methanolic extract of Acacia confusa heartwood and melanoxetin isolated from the extract. In: Holzforschung. 2015 ; Vol. 69, No. 5. pp. 645-652.
@article{37e04ccd12134926b75c5f94a8adb87f,
title = "Immune-regulatory activity of methanolic extract of Acacia confusa heartwood and melanoxetin isolated from the extract",
abstract = "The antioxidant, anti-inflammatory, antivirus, uric acid decreasing, and hepatoprotective activities of Acacia confusa extracts were demonstrated in previous studies. However, there is no scientific evidence concerning the immune-regulatory activity of the heartwood extract of A. confusa. In this study, the effect of a methanolic heartwood extract (MHE) from A. confusa on dendritic cell (DC) activation and function was examined. A. confusa MHE significantly reduced the production of pro-inflammatory cytokine interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated DCs, and the effective concentration (25 μg ml-1) of A. confusa MHE did not affect cell viability. Additionally, the bioactive phytochemical from A. confusa MHE, melanoxetin, was isolated and purified by HPLC. This substance inhibited the production of pro-inflammatory cytokines (tumor necrosis factor-α, IL-6, and IL-12) in LPS-stimulated DC at a concentration of 12.5 μM. Moreover, the expression levels of co-stimulatory molecules such as CD40, CD80, and CD86 also remarkably decreased after treatment with melanoxetin at the same dose. These findings indicate that A. confusa MHE and melanoxetin have excellent immune-suppressive activity and may be potential candidates for further development of natural health supplements.",
keywords = "Acacia confusa, dendritic cell, heartwood, immune-regulatory activity, melanoxetin",
author = "Ho, {Shang Tse} and Tung, {Yu Tang} and Wu, {Yu Jung} and Lin, {Chi Chen} and Wu, {Jyh Horng}",
year = "2015",
month = "7",
day = "1",
doi = "10.1515/hf-2014-0208",
language = "English",
volume = "69",
pages = "645--652",
journal = "Holzforschung",
issn = "0018-3830",
publisher = "Walter de Gruyter GmbH & Co. KG",
number = "5",

}

TY - JOUR

T1 - Immune-regulatory activity of methanolic extract of Acacia confusa heartwood and melanoxetin isolated from the extract

AU - Ho, Shang Tse

AU - Tung, Yu Tang

AU - Wu, Yu Jung

AU - Lin, Chi Chen

AU - Wu, Jyh Horng

PY - 2015/7/1

Y1 - 2015/7/1

N2 - The antioxidant, anti-inflammatory, antivirus, uric acid decreasing, and hepatoprotective activities of Acacia confusa extracts were demonstrated in previous studies. However, there is no scientific evidence concerning the immune-regulatory activity of the heartwood extract of A. confusa. In this study, the effect of a methanolic heartwood extract (MHE) from A. confusa on dendritic cell (DC) activation and function was examined. A. confusa MHE significantly reduced the production of pro-inflammatory cytokine interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated DCs, and the effective concentration (25 μg ml-1) of A. confusa MHE did not affect cell viability. Additionally, the bioactive phytochemical from A. confusa MHE, melanoxetin, was isolated and purified by HPLC. This substance inhibited the production of pro-inflammatory cytokines (tumor necrosis factor-α, IL-6, and IL-12) in LPS-stimulated DC at a concentration of 12.5 μM. Moreover, the expression levels of co-stimulatory molecules such as CD40, CD80, and CD86 also remarkably decreased after treatment with melanoxetin at the same dose. These findings indicate that A. confusa MHE and melanoxetin have excellent immune-suppressive activity and may be potential candidates for further development of natural health supplements.

AB - The antioxidant, anti-inflammatory, antivirus, uric acid decreasing, and hepatoprotective activities of Acacia confusa extracts were demonstrated in previous studies. However, there is no scientific evidence concerning the immune-regulatory activity of the heartwood extract of A. confusa. In this study, the effect of a methanolic heartwood extract (MHE) from A. confusa on dendritic cell (DC) activation and function was examined. A. confusa MHE significantly reduced the production of pro-inflammatory cytokine interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated DCs, and the effective concentration (25 μg ml-1) of A. confusa MHE did not affect cell viability. Additionally, the bioactive phytochemical from A. confusa MHE, melanoxetin, was isolated and purified by HPLC. This substance inhibited the production of pro-inflammatory cytokines (tumor necrosis factor-α, IL-6, and IL-12) in LPS-stimulated DC at a concentration of 12.5 μM. Moreover, the expression levels of co-stimulatory molecules such as CD40, CD80, and CD86 also remarkably decreased after treatment with melanoxetin at the same dose. These findings indicate that A. confusa MHE and melanoxetin have excellent immune-suppressive activity and may be potential candidates for further development of natural health supplements.

KW - Acacia confusa

KW - dendritic cell

KW - heartwood

KW - immune-regulatory activity

KW - melanoxetin

UR - http://www.scopus.com/inward/record.url?scp=84934280112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934280112&partnerID=8YFLogxK

U2 - 10.1515/hf-2014-0208

DO - 10.1515/hf-2014-0208

M3 - Article

VL - 69

SP - 645

EP - 652

JO - Holzforschung

JF - Holzforschung

SN - 0018-3830

IS - 5

ER -