Immobilization of antibody conjugated ZnS quantum dots onto poly(2,6-dimethyl-1,4-phenylene oxide) nanofibers with Poly(N-isopropylacrylamide) grafts as reversibly fluorescence immunoassay

Jian Wei Guo, Yi Hui Wu, Po Li Wei, Yan Jiun Huang, Jem Kun Chen

Research output: Contribution to journalArticle

Abstract

In this study a reversibly fluorescence immunoassay was prepared through grafting, using hydrogen bonding, of an antibody-conjugated ZnS quantum dots (ZQD-antiHA) onto an electrospun poly (2,6-dimethyl-1,4-phenylene oxide) (PPO) fibrous mats (EPFM) with poly (n-isopropylacrylamide) (PNIPAAm) grafts. The PPO was first electrospun into a fibrous mat, then brominated and reacted with NaN3 to generate the azido-terminated EPFMs. A propargyl-terminated poly (n-isopropylacrylamide) (PNIPAAm) was synthesized and grafted onto the azido-terminated EPFMs. ZQDs were conjugated by antibodies (antiHA) to adsorb onto the PNIPAAm grafts of EPFMs with hydrogen bonding. Because of the stronger interaction between antigen (HA) and antibody (antiHA), the hydrogen bonding was blocked due to the introduction of the antigen which resulted in the desorption the ZQDs from the EPFMs. The blue fluorescence of the PNIPAAm-grafted EPFMs with antibody-conjugated ZQDs was disappeared while immersing in the solution of antigen. The 3.82 mg/m2 of ZQDs on the PNIPAAm grafts EPFMs significantly reduced to 0.22 mg/m2 after the immersion of the antigen solution which verified the transference of the ZQDs from EPFMs to the solution. The behavior of adsorption and desorption of the ZQDs was reversible up to five cycles. The revisable fluorescence immunoassay of EPFMs with PNIPAAm grafts experimentally exhibited a high potential in a simple setup for biosensing with its unique sensitivity and selectivity.

Original languageEnglish
Pages (from-to)198-208
Number of pages11
JournalDyes and Pigments
Volume159
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Antigens
Nanofibers
Antibodies
Grafts
Semiconductor quantum dots
Fluorescence
Polyphenylene oxides
Hydrogen bonds
Oxides
Desorption
Sodium Azide
Adsorption
poly-N-isopropylacrylamide
poly(2,6-dimethyl-1,4-phenylene oxide)

Keywords

  • Click reaction
  • Immunoassay
  • poly(N-isopropylacrylamide)
  • poly(phenylene oxide)

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Process Chemistry and Technology

Cite this

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title = "Immobilization of antibody conjugated ZnS quantum dots onto poly(2,6-dimethyl-1,4-phenylene oxide) nanofibers with Poly(N-isopropylacrylamide) grafts as reversibly fluorescence immunoassay",
abstract = "In this study a reversibly fluorescence immunoassay was prepared through grafting, using hydrogen bonding, of an antibody-conjugated ZnS quantum dots (ZQD-antiHA) onto an electrospun poly (2,6-dimethyl-1,4-phenylene oxide) (PPO) fibrous mats (EPFM) with poly (n-isopropylacrylamide) (PNIPAAm) grafts. The PPO was first electrospun into a fibrous mat, then brominated and reacted with NaN3 to generate the azido-terminated EPFMs. A propargyl-terminated poly (n-isopropylacrylamide) (PNIPAAm) was synthesized and grafted onto the azido-terminated EPFMs. ZQDs were conjugated by antibodies (antiHA) to adsorb onto the PNIPAAm grafts of EPFMs with hydrogen bonding. Because of the stronger interaction between antigen (HA) and antibody (antiHA), the hydrogen bonding was blocked due to the introduction of the antigen which resulted in the desorption the ZQDs from the EPFMs. The blue fluorescence of the PNIPAAm-grafted EPFMs with antibody-conjugated ZQDs was disappeared while immersing in the solution of antigen. The 3.82 mg/m2 of ZQDs on the PNIPAAm grafts EPFMs significantly reduced to 0.22 mg/m2 after the immersion of the antigen solution which verified the transference of the ZQDs from EPFMs to the solution. The behavior of adsorption and desorption of the ZQDs was reversible up to five cycles. The revisable fluorescence immunoassay of EPFMs with PNIPAAm grafts experimentally exhibited a high potential in a simple setup for biosensing with its unique sensitivity and selectivity.",
keywords = "Click reaction, Immunoassay, poly(N-isopropylacrylamide), poly(phenylene oxide)",
author = "Guo, {Jian Wei} and Wu, {Yi Hui} and Wei, {Po Li} and Huang, {Yan Jiun} and Chen, {Jem Kun}",
year = "2018",
month = "12",
day = "1",
doi = "10.1016/j.dyepig.2018.05.059",
language = "English",
volume = "159",
pages = "198--208",
journal = "Dyes and Pigments",
issn = "0143-7208",
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T1 - Immobilization of antibody conjugated ZnS quantum dots onto poly(2,6-dimethyl-1,4-phenylene oxide) nanofibers with Poly(N-isopropylacrylamide) grafts as reversibly fluorescence immunoassay

AU - Guo, Jian Wei

AU - Wu, Yi Hui

AU - Wei, Po Li

AU - Huang, Yan Jiun

AU - Chen, Jem Kun

PY - 2018/12/1

Y1 - 2018/12/1

N2 - In this study a reversibly fluorescence immunoassay was prepared through grafting, using hydrogen bonding, of an antibody-conjugated ZnS quantum dots (ZQD-antiHA) onto an electrospun poly (2,6-dimethyl-1,4-phenylene oxide) (PPO) fibrous mats (EPFM) with poly (n-isopropylacrylamide) (PNIPAAm) grafts. The PPO was first electrospun into a fibrous mat, then brominated and reacted with NaN3 to generate the azido-terminated EPFMs. A propargyl-terminated poly (n-isopropylacrylamide) (PNIPAAm) was synthesized and grafted onto the azido-terminated EPFMs. ZQDs were conjugated by antibodies (antiHA) to adsorb onto the PNIPAAm grafts of EPFMs with hydrogen bonding. Because of the stronger interaction between antigen (HA) and antibody (antiHA), the hydrogen bonding was blocked due to the introduction of the antigen which resulted in the desorption the ZQDs from the EPFMs. The blue fluorescence of the PNIPAAm-grafted EPFMs with antibody-conjugated ZQDs was disappeared while immersing in the solution of antigen. The 3.82 mg/m2 of ZQDs on the PNIPAAm grafts EPFMs significantly reduced to 0.22 mg/m2 after the immersion of the antigen solution which verified the transference of the ZQDs from EPFMs to the solution. The behavior of adsorption and desorption of the ZQDs was reversible up to five cycles. The revisable fluorescence immunoassay of EPFMs with PNIPAAm grafts experimentally exhibited a high potential in a simple setup for biosensing with its unique sensitivity and selectivity.

AB - In this study a reversibly fluorescence immunoassay was prepared through grafting, using hydrogen bonding, of an antibody-conjugated ZnS quantum dots (ZQD-antiHA) onto an electrospun poly (2,6-dimethyl-1,4-phenylene oxide) (PPO) fibrous mats (EPFM) with poly (n-isopropylacrylamide) (PNIPAAm) grafts. The PPO was first electrospun into a fibrous mat, then brominated and reacted with NaN3 to generate the azido-terminated EPFMs. A propargyl-terminated poly (n-isopropylacrylamide) (PNIPAAm) was synthesized and grafted onto the azido-terminated EPFMs. ZQDs were conjugated by antibodies (antiHA) to adsorb onto the PNIPAAm grafts of EPFMs with hydrogen bonding. Because of the stronger interaction between antigen (HA) and antibody (antiHA), the hydrogen bonding was blocked due to the introduction of the antigen which resulted in the desorption the ZQDs from the EPFMs. The blue fluorescence of the PNIPAAm-grafted EPFMs with antibody-conjugated ZQDs was disappeared while immersing in the solution of antigen. The 3.82 mg/m2 of ZQDs on the PNIPAAm grafts EPFMs significantly reduced to 0.22 mg/m2 after the immersion of the antigen solution which verified the transference of the ZQDs from EPFMs to the solution. The behavior of adsorption and desorption of the ZQDs was reversible up to five cycles. The revisable fluorescence immunoassay of EPFMs with PNIPAAm grafts experimentally exhibited a high potential in a simple setup for biosensing with its unique sensitivity and selectivity.

KW - Click reaction

KW - Immunoassay

KW - poly(N-isopropylacrylamide)

KW - poly(phenylene oxide)

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U2 - 10.1016/j.dyepig.2018.05.059

DO - 10.1016/j.dyepig.2018.05.059

M3 - Article

AN - SCOPUS:85048800871

VL - 159

SP - 198

EP - 208

JO - Dyes and Pigments

JF - Dyes and Pigments

SN - 0143-7208

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