Imaging serotonin transporters with 123I-ADAM brain SPECT in healthy non-human primates

Wen Sheng Huang, Kuo Hsing Ma, Cheng Yi Cheng, Cheng Yu Chen, Ying Kai Fu, Yuan Hwa Chou, Shiaw Pyng Wey, Jian Chuan Liu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Serotonin transporters (SERTs) play a major role in modulating serotonergic neuronal function and are the target of many antidepressant drugs used in neuro-psychiatric disorders. To gain more information on the temporal distribution of SERTs, 2-([2-([dimethylamino]methyl)phenoxyl]thio)-5-[ 123I] iodophenylamine (123I-ADAM) single photon emission computed tomography (SPECT) was utilized in an in vivo imaging study using non-human primates. Methods: Two female monkeys (Macaca cyclopis) were studied. Eight brain SPECT imaging examinations, each 30 min in duration, were obtained after injection of 185 MBq of 123I-ADAM. Images were obtained using a dual-head gamma camera equipped with ultra-high resolution fan-beam collimators. In addition to visual inspection, the radio-uptake and specific uptake ratios (SURs) of midbrain (MB), thalamus (TH), striatum (ST), temporal and frontal cortices and the whole brain in reference to the corresponding magnetic resonance image at the eight time points were measured. The SUR of MB, using cerebellum (CB) as the reference tissue, was calculated as (MB - CB)/CB, in mean counts/pixel. The SURs of the other brain regions were similarly measured. Results: There was relatively high uptake of 123I-ADAM in the MB and TH, moderate uptake in ST, lower uptake in the cerebral cortex, and almost no uptake in the CB. The image of MB could be easily identified at the first 30 min time point. It appeared that the SURs of MB, TH and ST reached equilibrium around 210 min after injection. No adverse reactions of the primates were found during and after imaging. Brain distribution of 123I-ADAM in the primate appeared consistent with the known distribution of SERTs. Conclusion: In conjunction with a high SUR in MB, TH and ST, we speculate that 123I-ADAM may be a potential radioligand for SPECT studies of serotonin transporters in humans.

Original languageEnglish
Pages (from-to)515-519
Number of pages5
JournalNuclear Medicine Communications
Volume25
Issue number5
DOIs
Publication statusPublished - May 2004
Externally publishedYes

Fingerprint

Serotonin Plasma Membrane Transport Proteins
Mesencephalon
Single-Photon Emission-Computed Tomography
Primates
Thalamus
Brain
Cerebellum
Injections
Gamma Cameras
Macaca
Frontal Lobe
Temporal Lobe
2-((2-((dimethylamino)methyl)phenyl)thio)-5-iodophenylamine
Radio
Cerebral Cortex
Antidepressive Agents
Haplorhini
Psychiatry
Magnetic Resonance Spectroscopy
Head

Keywords

  • I-ADAM
  • Brain SPECT
  • Non-human primate
  • Serotonin transporter

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Imaging serotonin transporters with 123I-ADAM brain SPECT in healthy non-human primates. / Huang, Wen Sheng; Ma, Kuo Hsing; Cheng, Cheng Yi; Chen, Cheng Yu; Fu, Ying Kai; Chou, Yuan Hwa; Wey, Shiaw Pyng; Liu, Jian Chuan.

In: Nuclear Medicine Communications, Vol. 25, No. 5, 05.2004, p. 515-519.

Research output: Contribution to journalArticle

Huang, Wen Sheng ; Ma, Kuo Hsing ; Cheng, Cheng Yi ; Chen, Cheng Yu ; Fu, Ying Kai ; Chou, Yuan Hwa ; Wey, Shiaw Pyng ; Liu, Jian Chuan. / Imaging serotonin transporters with 123I-ADAM brain SPECT in healthy non-human primates. In: Nuclear Medicine Communications. 2004 ; Vol. 25, No. 5. pp. 515-519.
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AU - Fu, Ying Kai

AU - Chou, Yuan Hwa

AU - Wey, Shiaw Pyng

AU - Liu, Jian Chuan

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