Imaging receptor microdomains on leukocyte subsets in live mice

Elaine Y. Chiang, Andrés Hidalgo, Jungshan Chang, Paul S. Frenette

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-α-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalNature Methods
Volume4
Issue number3
DOIs
Publication statusPublished - Mar 2007
Externally publishedYes

Fingerprint

Venules
Leukocytes
Imaging techniques
Lymphocyte Function-Associated Antigen-1
Lymphocytes
Surgery
Endothelium
Cluster Analysis
Bone
Neutrophils
B-Lymphocytes
Bone Marrow
Polarization

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Imaging receptor microdomains on leukocyte subsets in live mice. / Chiang, Elaine Y.; Hidalgo, Andrés; Chang, Jungshan; Frenette, Paul S.

In: Nature Methods, Vol. 4, No. 3, 03.2007, p. 219-222.

Research output: Contribution to journalArticle

Chiang, Elaine Y. ; Hidalgo, Andrés ; Chang, Jungshan ; Frenette, Paul S. / Imaging receptor microdomains on leukocyte subsets in live mice. In: Nature Methods. 2007 ; Vol. 4, No. 3. pp. 219-222.
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