Imaging of single liver tumor cells intoxicated by heavy metals using ToF-SIMS

Fu Der Mai, Bo Jung Chen, Li Chen Wu, Feng Yin Li, Wen Kang Chen

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Human liver tumor cells intoxicated with five different Cd, Cu, Cr, Hg and Zn metals were analyzed using imaging time-of-flight secondary ion mass spectrometry (ToF-SIMS) to visualize the metal distributions in a single cell basis. A protocol was developed by combining rapid freezing, freeze-fracture and imprinting for transferring the intoxicated cells to a silicon wafer. As shown in the ToF-SIMS images, the cellular morphology was preserved indicating that this protocol can be used to prepare a representative cell for ToF-SIMS imaging analysis. Among the five metal ions investigated in this study, only Cr and Cu ions show preferential diffusion into the cell after simulated intoxication while the signals of the other three ions are either too low to be detected or unable to be distinguished from background intensity.

Original languageEnglish
Pages (from-to)6809-6812
Number of pages4
JournalApplied Surface Science
Volume252
Issue number19
DOIs
Publication statusPublished - Jul 30 2006
Externally publishedYes

Fingerprint

heavy metals
Secondary ion mass spectrometry
Heavy Metals
liver
Liver
secondary ion mass spectrometry
Heavy metals
Tumors
tumors
Cells
Imaging techniques
Metals
cells
Ions
intoxication
Silicon wafers
Freezing
Metal ions
metals
freezing

Keywords

  • Heavy metals
  • Imaging
  • Liver cancer
  • ToF-SIMS
  • Tumor cells

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Condensed Matter Physics

Cite this

Imaging of single liver tumor cells intoxicated by heavy metals using ToF-SIMS. / Mai, Fu Der; Chen, Bo Jung; Wu, Li Chen; Li, Feng Yin; Chen, Wen Kang.

In: Applied Surface Science, Vol. 252, No. 19, 30.07.2006, p. 6809-6812.

Research output: Contribution to journalArticle

Mai, Fu Der ; Chen, Bo Jung ; Wu, Li Chen ; Li, Feng Yin ; Chen, Wen Kang. / Imaging of single liver tumor cells intoxicated by heavy metals using ToF-SIMS. In: Applied Surface Science. 2006 ; Vol. 252, No. 19. pp. 6809-6812.
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