IL-13 expression at the sites of allergen challenge in patients with asthma

S. K. Huang, H. Q. Xiao, J. Kleine-Tebbe, G. Paciotti, D. G. Marsh, L. M. Lichtenstein, M. C. Liu

Research output: Contribution to journalArticle

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Abstract

Atopic asthma is characterized by inflammatory responses of the airway and is associated with up-regulation of Th2 cytokines, notably IL-4 and IL-5. A recently described human cytokine, IL-13, is a potent in vitro modulator of various cell types, including monocytes, B cells, and endothelial cells. Similar to IL-4, it is also involved in the induction of IgE synthesis. However, the in vivo expression and function of IL-13 and its relation to disease remain to be defined. Using a segmental allergen challenge model, we have examined the in vivo expression of IL-13 in the bronchoalveolar lavage (BAL) cells of atopic patients. We found a significant enhancement of both IL-13 transcripts and secreted proteins in the allergen-challenged BAL compared with the saline-challenged control sites of asthmatic and rhinitic patients. In contrast, the expression of IL-13 transcripts was not detected in the BAL of two normal subjects challenged with the same dose of ragweed allergen. The cellular source of IL-13 mRNA was identified in the mononuclear cell fraction of the allergen-challenged BAL. The allergen-induced quantitative differences in the level of transcripts were confirmed by competitive PCR assays. These results suggest that the significant increase in IL-13 in the allergen-challenged BAL is primarily from the mononuclear cells and is involved in the regulation of allergen-induced late phase inflammatory responses.

Original languageEnglish
Pages (from-to)2688-2694
Number of pages7
JournalJournal of Immunology
Volume155
Issue number5
Publication statusPublished - Jan 1 1995
Externally publishedYes

Fingerprint

Interleukin-13
Allergens
Asthma
Bronchoalveolar Lavage
Interleukin-4
Cytokines
Ambrosia
Interleukin-5
Immunoglobulin E
Monocytes
B-Lymphocytes
Up-Regulation
Endothelial Cells
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Huang, S. K., Xiao, H. Q., Kleine-Tebbe, J., Paciotti, G., Marsh, D. G., Lichtenstein, L. M., & Liu, M. C. (1995). IL-13 expression at the sites of allergen challenge in patients with asthma. Journal of Immunology, 155(5), 2688-2694.

IL-13 expression at the sites of allergen challenge in patients with asthma. / Huang, S. K.; Xiao, H. Q.; Kleine-Tebbe, J.; Paciotti, G.; Marsh, D. G.; Lichtenstein, L. M.; Liu, M. C.

In: Journal of Immunology, Vol. 155, No. 5, 01.01.1995, p. 2688-2694.

Research output: Contribution to journalArticle

Huang, SK, Xiao, HQ, Kleine-Tebbe, J, Paciotti, G, Marsh, DG, Lichtenstein, LM & Liu, MC 1995, 'IL-13 expression at the sites of allergen challenge in patients with asthma', Journal of Immunology, vol. 155, no. 5, pp. 2688-2694.
Huang SK, Xiao HQ, Kleine-Tebbe J, Paciotti G, Marsh DG, Lichtenstein LM et al. IL-13 expression at the sites of allergen challenge in patients with asthma. Journal of Immunology. 1995 Jan 1;155(5):2688-2694.
Huang, S. K. ; Xiao, H. Q. ; Kleine-Tebbe, J. ; Paciotti, G. ; Marsh, D. G. ; Lichtenstein, L. M. ; Liu, M. C. / IL-13 expression at the sites of allergen challenge in patients with asthma. In: Journal of Immunology. 1995 ; Vol. 155, No. 5. pp. 2688-2694.
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