IGF-I secretion by prostate carcinoma cells does not alter tumor-bone cell interactions in vitro or in vivo

Janet Rubin, Xian Fan, Jill Rahnert, Buer Sen, Chia Ling Hsieh, Tamara C. Murphy, Mark S. Nanes, Lindsay G. Horton, Wesley G. Beamer, Clifford J. Rosen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

BACKGROUND. IGF-I is an important growth and differentiative factor for osteoblasts and may have a role in defining prostate cancer risk and skeletal metastases. METHODS. Conditioned media (CM) from human prostate cancer (PC), C4-2 and C4-2B, which produce osteoblastic lesions, and PC-3, which causes osteolysis, was added to MC3T3-E1 bone cultures. SCID mice were injected intratibially with these engineered cells. Tumor bearing tibiae were analyzed by microCT and pQCT. RESULTS. CM from PC cells increased osteoblast proliferation and differentiation and was unaltered by the type of PC cell, IGF-I antibodies, or exogenous IGF-I and IGFBP2. Study of intratibial PC tumors in SCID mice showed that C4-2 cells grew slowly preserving bone structure, while PC-3 tumors caused rapid osteolysis. Overexpression of IGF-I did not change either tumor progression or skeletal response. CONCLUSIONS. IGF-I is neither necessary nor sufficient for the osteoblastic response to PC metastases.

Original languageEnglish
Pages (from-to)789-800
Number of pages12
JournalProstate
Volume66
Issue number8
DOIs
Publication statusPublished - Jun 1 2006
Externally publishedYes

Keywords

  • LNCaP
  • Osteoblast
  • Osteolysis
  • PC-3

ASJC Scopus subject areas

  • Oncology
  • Urology

Fingerprint Dive into the research topics of 'IGF-I secretion by prostate carcinoma cells does not alter tumor-bone cell interactions in vitro or in vivo'. Together they form a unique fingerprint.

  • Cite this

    Rubin, J., Fan, X., Rahnert, J., Sen, B., Hsieh, C. L., Murphy, T. C., Nanes, M. S., Horton, L. G., Beamer, W. G., & Rosen, C. J. (2006). IGF-I secretion by prostate carcinoma cells does not alter tumor-bone cell interactions in vitro or in vivo. Prostate, 66(8), 789-800. https://doi.org/10.1002/pros.20379