Identification of the chitinase genes from the diamondback moth, Plutella xylostella

Z. H. Liao, T. C. Kuo, C. H. Kao, T. M. Chou, Y. H. Kao, R. N. Huang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Chitinases have an indispensable function in chitin metabolism and are well characterized in numerous insect species. Although the diamondback moth (DBM) Plutella xylostella, which has a high reproductive potential, short generation time, and characteristic adaptation to adverse environments, has become one of the most serious pests of cruciferous plants worldwide, the information on the chitinases of the moth is presently limited. In the present study, using degenerated polymerase chain reaction (PCR) and rapid amplification of cDNA ends-PCR strategies, four chitinase genes of P. xylostella were cloned, and an exhaustive search was conducted for chitinase-like sequences from the P. xylostella genome and transcriptomic database. Based on the domain analysis of the deduced amino acid sequences and the phylogenetic analysis of the catalytic domain sequences, we identified 15 chitinase genes from P. xylostella. Two of the gut-specific chitinases did not cluster with any of the known phylogenetic groups of chitinases and might be in a new group of the chitinase family. Moreover, in our study, group VIII chitinase was not identified. The structures, classifications and expression patterns of the chitinases of P. xylostella were further delineated, and with this information, further investigations on the functions of chitinase genes in DBM could be facilitated.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalBulletin of Entomological Research
DOIs
Publication statusAccepted/In press - Jul 15 2016

Fingerprint

Plutella xylostella
chitinase
genes
polymerase chain reaction
plant pests
rapid amplification of cDNA ends
phylogeny
chitin
transcriptomics
active sites
moths
amino acid sequences
digestive system
taxonomy

Keywords

  • chitinase
  • phylogenesis
  • Plutella xylostella

ASJC Scopus subject areas

  • Insect Science
  • Agronomy and Crop Science

Cite this

Identification of the chitinase genes from the diamondback moth, Plutella xylostella. / Liao, Z. H.; Kuo, T. C.; Kao, C. H.; Chou, T. M.; Kao, Y. H.; Huang, R. N.

In: Bulletin of Entomological Research, 15.07.2016, p. 1-12.

Research output: Contribution to journalArticle

Liao, Z. H. ; Kuo, T. C. ; Kao, C. H. ; Chou, T. M. ; Kao, Y. H. ; Huang, R. N. / Identification of the chitinase genes from the diamondback moth, Plutella xylostella. In: Bulletin of Entomological Research. 2016 ; pp. 1-12.
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