Identification of synthetic vaccine candidates against SARS CoV infection

Shu Pei Lien, Yi Ping Shih, Hsin Wei Chen, Jy Ping Tsai, Chih Hsiang Leng, Min Han Lin, Li Hsiu Lin, Hsin Yu Liu, Ai Hsiang Chou, Yu Wen Chang, Yi Ming A. Chen, Pele Chong, Shih Jen Liu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Three peptides, D1 (amino acid residues 175-201), D2 (a.a. 434-467), and TM (a.a. 1128-1159), corresponding to the spike protein (S) of severe acute respiratory syndrome corona virus (SARS CoV) were synthesized and their immunological functions were investigated in three different animals models (mice, guinea pigs, and rabbits). The peptides mixture formulated either with Freund's adjuvant or synthetic adjuvant Montanide ISA-51/oligodeoxy nucleotide CpG (ISA/CpG) could elicit antisera in immunized animals which were capable of inhibiting SARS/HIV pseudovirus entry into HepG2 cells. The neutralizing epitopes were identified using peptides to block the neutralizing effect of guinea pig antisera. The major neutralizing epitope was located on the D2 peptide, and the amino acid residue was fine mapped to 434-453. In BALB/c mice T-cell proliferation assay revealed that only D2 peptide contained T-cell epitope, the sequence of which corresponded to amino acid residue 434-448. The ISA/CpG formulation generated anti-D2 IgG titer comparable to those obtained from Freund's adjuvant formulation, but generated fewer antibodies against D1 or TM peptides. The highly immunogenic D2 peptide contains both neutralizing and Th cell epitopes. These results suggest that synthetic peptide D2 would be useful as a component of SARS vaccine candidates.

Original languageEnglish
Pages (from-to)716-721
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume358
Issue number3
DOIs
Publication statusPublished - Jul 6 2007
Externally publishedYes

Keywords

  • Epitopes
  • Peptide
  • Pseudovirus
  • SARS CoV
  • Spike protein
  • Vaccine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Lien, S. P., Shih, Y. P., Chen, H. W., Tsai, J. P., Leng, C. H., Lin, M. H., Lin, L. H., Liu, H. Y., Chou, A. H., Chang, Y. W., Chen, Y. M. A., Chong, P., & Liu, S. J. (2007). Identification of synthetic vaccine candidates against SARS CoV infection. Biochemical and Biophysical Research Communications, 358(3), 716-721. https://doi.org/10.1016/j.bbrc.2007.04.164