Identification of phosphorylated cyclin-dependent kinase 1 associated with colorectal cancer survival using label-free quantitative analyses

Peng Chan Lin, Yi Fang Yang, Yu Chang Tyan, Eric S.L. Hsiao, Po Chen Chu, Chung Ta Lee, Jenq Chang Lee, Yi Ming Arthur Chen, Pao Chi Liao

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Colorectal cancer is the most common form of cancer in the world, and the five-year survival rate is estimated to be almost 90% in the early stages. Therefore, the identification of potential biomarkers to assess the prognosis of early stage colorectal cancer patients is critical for further clinical treatment. Dysregulated tyrosine phosphorylation has been found in several diseases that play a significant regulator of signaling in cellular pathways. In this study, this strategy was used to characterize the tyrosine phosphoproteome of colorectal cell lines with different progression abilities (SW480 and SW620). We identified a total of 280 phosphotyrosine (pTyr) peptides comprising 287 pTyr sites from 261 proteins. Label-free quantitative analysis revealed the differential level of a total of 103 pTyr peptides between SW480 and SW620 cells. We showed that cyclin-dependent kinase I (CDK1) pTyr15 level in SW480 cells was 3.3-fold greater than in SW620 cells, and these data corresponded with the label-free mass spectrometry-based proteomic quantification analysis. High level CDK1 pTyr15 was associated with prolonged disease-free survival for stage II colorectal cancer patients (n = 79). Taken together, our results suggest that the CDK1 pTyr15 protein is a potential indicator of the progression of colorectal cancer.

Original languageEnglish
Article numbere0158844
JournalPLoS One
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 1 2016
Externally publishedYes

Fingerprint

CDC2 Protein Kinase
Phosphotyrosine
cyclin-dependent kinase
colorectal neoplasms
Labels
Colorectal Neoplasms
Survival
Tyrosine
tyrosine
Peptides
Phosphorylation
Cyclin-Dependent Kinases
Biomarkers
peptides
cell communication
Mass spectrometry
Proteins
cells
Cells
Proteomics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Identification of phosphorylated cyclin-dependent kinase 1 associated with colorectal cancer survival using label-free quantitative analyses. / Lin, Peng Chan; Yang, Yi Fang; Tyan, Yu Chang; Hsiao, Eric S.L.; Chu, Po Chen; Lee, Chung Ta; Lee, Jenq Chang; Chen, Yi Ming Arthur; Liao, Pao Chi.

In: PLoS One, Vol. 11, No. 7, e0158844, 01.07.2016.

Research output: Contribution to journalArticle

Lin, Peng Chan ; Yang, Yi Fang ; Tyan, Yu Chang ; Hsiao, Eric S.L. ; Chu, Po Chen ; Lee, Chung Ta ; Lee, Jenq Chang ; Chen, Yi Ming Arthur ; Liao, Pao Chi. / Identification of phosphorylated cyclin-dependent kinase 1 associated with colorectal cancer survival using label-free quantitative analyses. In: PLoS One. 2016 ; Vol. 11, No. 7.
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