Identification of oral carcinogenesis using autofluorescence spectroscopy: An in vivo study

Tsui Min Tsai, Chin Tin Chen, Chih Yu Wang, Chun Pin Chiang, Yu Luan Kuo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A study on in vivo measurement of autofluorescence spectra for hamster buccal pouch and development of oral carcinogenesis identification algorithm is presented. The measurement was preceded with a fiber-optics based fluorescence spectroscopy system. In total 75 samples, including 14 hyperkeratosis, 23 normal, 28 dysplasia, and 10 SCC, were separated into 4 categories. All the spectra were normalized to have the same area below the spectrum curve. The results show that the autofluorescence spectra start to change as soon as the tissues have morphological alternation (eg hyperkeratosis). The differences of ratios between the areas under 380 ± 15 nm and 460 ± 15 nm (denoted as A380±15 / A460±15) among categories are statistically significant. To develop a diagnostic algorithm for early neoplasia detection and evaluate its performance, a PLS discriminant analysis with cross-validation technique was proceeded. Sample points on the PLS score plot were grouped as four categories. By selecting suitable threshold, the accuracy rates for classifying 4 categories of samples are 86% (hyperkeratosis), 87% (normal), 90% (dysplasia), and 100% (SCC), respectively. The results reveal that the autofluorescence spectroscopy technique is potential for in vivo detection of early neoplasia of oral tissues.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsA.E.T. Chiou, H. Podbielska, S.L. Jacques
Pages115-120
Number of pages6
Volume4597
DOIs
Publication statusPublished - 2001
EventBiophotonics Instumentation and Analysis - Singapore, Singapore
Duration: Nov 28 2001Nov 29 2001

Other

OtherBiophotonics Instumentation and Analysis
CountrySingapore
CitySingapore
Period11/28/0111/29/01

Fingerprint

Spectroscopy
Tissue
Fluorescence spectroscopy
Discriminant analysis
spectroscopy
Fiber optics
hamsters
alternations
classifying
fiber optics
plots
fluorescence
thresholds
curves

Keywords

  • Autofluorescence spectroscopy
  • In vivo measurement
  • Oral carcinogenesis

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Tsai, T. M., Chen, C. T., Wang, C. Y., Chiang, C. P., & Kuo, Y. L. (2001). Identification of oral carcinogenesis using autofluorescence spectroscopy: An in vivo study. In A. E. T. Chiou, H. Podbielska, & S. L. Jacques (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4597, pp. 115-120) https://doi.org/10.1117/12.446646

Identification of oral carcinogenesis using autofluorescence spectroscopy : An in vivo study. / Tsai, Tsui Min; Chen, Chin Tin; Wang, Chih Yu; Chiang, Chun Pin; Kuo, Yu Luan.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / A.E.T. Chiou; H. Podbielska; S.L. Jacques. Vol. 4597 2001. p. 115-120.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsai, TM, Chen, CT, Wang, CY, Chiang, CP & Kuo, YL 2001, Identification of oral carcinogenesis using autofluorescence spectroscopy: An in vivo study. in AET Chiou, H Podbielska & SL Jacques (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4597, pp. 115-120, Biophotonics Instumentation and Analysis, Singapore, Singapore, 11/28/01. https://doi.org/10.1117/12.446646
Tsai TM, Chen CT, Wang CY, Chiang CP, Kuo YL. Identification of oral carcinogenesis using autofluorescence spectroscopy: An in vivo study. In Chiou AET, Podbielska H, Jacques SL, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4597. 2001. p. 115-120 https://doi.org/10.1117/12.446646
Tsai, Tsui Min ; Chen, Chin Tin ; Wang, Chih Yu ; Chiang, Chun Pin ; Kuo, Yu Luan. / Identification of oral carcinogenesis using autofluorescence spectroscopy : An in vivo study. Proceedings of SPIE - The International Society for Optical Engineering. editor / A.E.T. Chiou ; H. Podbielska ; S.L. Jacques. Vol. 4597 2001. pp. 115-120
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