Identification of Illegal Coal Tar Dyes Constituents in Mucous Cosmetics by HPLC Method

Kun Shan Wu, An Bang Wu, Ming Chuan Huang, Chau Yang Chen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Illegal coal tar dyes are reported to possess strong toxicity and carcinogenecity when added in mucous cosmetics. According to CNS, current methods in analyzing organic coloring materials (dyes) are mainly filter paper chromatography and thin-layer chromatography. In this study, we adopted a refined HPLC procedure reaching optimal conditions for the separation and identification of the coloring constituents allegedly added in mucous cosmetics. Ultimately, the analytical efficiency and precision are greatly improved. The optimal conditions for the HPLC method were found with a column of Cosmosil 5C18-AR-II; with the first mobile phase using 0.07 M ammonium acetate solution in 0.01 M tetrabutylammonium bromide (CH3COONH4) : CH3CN : CH3OH = 55:35:10 for analyzing 16 hydrophilic coal tar dyes, while the second mobile phase uses CH3OH : H2O = 95:5 for analyzing 6 hydrophobic dyes and a photodiode array detector within UV-VIS wavelength. By applying this HPLC method, a total of 22 banned coal tar dye constituents can be satisfactorily separated. It is also found that the detection limit can be improved to 0.05 μg/mL.

Original languageEnglish
Pages (from-to)95-105
Number of pages11
JournalJournal of Food and Drug Analysis
Volume7
Issue number2
Publication statusPublished - Jun 1999

Fingerprint

Coal Tar
cosmetics
Cosmetics
dyes
Coloring Agents
high performance liquid chromatography
High Pressure Liquid Chromatography
Paper Chromatography
paper chromatography
methodology
ammonium acetate
Thin Layer Chromatography
bromides
thin layer chromatography
detectors
Limit of Detection
wavelengths
detection limit
coal tar
toxicity

Keywords

  • High performance liquid chromatography (HPLC)
  • Illegal coal tar dyes
  • Mucous cosmetics

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

Identification of Illegal Coal Tar Dyes Constituents in Mucous Cosmetics by HPLC Method. / Wu, Kun Shan; Wu, An Bang; Huang, Ming Chuan; Chen, Chau Yang.

In: Journal of Food and Drug Analysis, Vol. 7, No. 2, 06.1999, p. 95-105.

Research output: Contribution to journalArticle

Wu, Kun Shan ; Wu, An Bang ; Huang, Ming Chuan ; Chen, Chau Yang. / Identification of Illegal Coal Tar Dyes Constituents in Mucous Cosmetics by HPLC Method. In: Journal of Food and Drug Analysis. 1999 ; Vol. 7, No. 2. pp. 95-105.
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