Identification of equine lactadherin-derived peptides that inhibit rotavirus infection via integrin receptor competition

Andrea Civra, Maria Gabriella Giuffrida, Manuela Donalisio, Lorenzo Napolitano, Yoshikazu Takada, Barbara S. Coulson, Amedeo Conti, David Lembo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the age of 5 years in both developed and developing countries. Human lactadherin, a milk fat globule membrane glycoprotein, inhibits human rotavirus infection in vitro, whereas bovine lactadherin is not active. Moreover, it protects breastfed infants against symptomatic rotavirus infections. To explore the potential antiviral activity of lactadherin sourced by equines, we undertook a proteomic analysis of milk fat globule membrane proteins from donkey milk and elucidated its amino acid sequence. Alignment of the human, bovine, and donkey lactadherin sequences revealed the presence of an Asp-Gly-Glu (DGE) α2β1 integrin-binding motif in the N-terminal domain of donkey sequence only. Because integrin α2β1 plays a critical role during early steps of rotavirus host cell adhesion, we tested a minilibrary of donkey lactadherin-derived peptides containing DGE sequence for anti-rotavirus activity. A 20-amino acid peptide containing both DGE and RGD motifs (named pDGE-RGD) showed the greatest activity, and its mechanism of antiviral action was characterized; pDGE-RGD binds to integrin α2β1 by means of the DGE motif and inhibits rotavirus attachment to the cell surface. These findings suggest the potential anti-rotavirus activity of equine lactadherin and support the feasibility of developing an anti-rotavirus peptide that acts by hindering virus-receptor binding.

Original languageEnglish
Pages (from-to)12403-12414
Number of pages12
JournalJournal of Biological Chemistry
Volume290
Issue number19
DOIs
Publication statusPublished - May 8 2015
Externally publishedYes

Fingerprint

Rotavirus Infections
Rotavirus
Integrins
Horses
Equidae
Peptides
Antiviral Agents
Virus Receptors
Amino Acids
Cell adhesion
Membrane Glycoproteins
aspartyl-glycyl-glutamyl-alanine
Developing countries
Membrane Proteins
Virus Attachment
Gastroenteritis
Developed Countries
Cell Adhesion
Proteomics
Developing Countries

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Civra, A., Giuffrida, M. G., Donalisio, M., Napolitano, L., Takada, Y., Coulson, B. S., ... Lembo, D. (2015). Identification of equine lactadherin-derived peptides that inhibit rotavirus infection via integrin receptor competition. Journal of Biological Chemistry, 290(19), 12403-12414. https://doi.org/10.1074/jbc.M114.620500

Identification of equine lactadherin-derived peptides that inhibit rotavirus infection via integrin receptor competition. / Civra, Andrea; Giuffrida, Maria Gabriella; Donalisio, Manuela; Napolitano, Lorenzo; Takada, Yoshikazu; Coulson, Barbara S.; Conti, Amedeo; Lembo, David.

In: Journal of Biological Chemistry, Vol. 290, No. 19, 08.05.2015, p. 12403-12414.

Research output: Contribution to journalArticle

Civra, A, Giuffrida, MG, Donalisio, M, Napolitano, L, Takada, Y, Coulson, BS, Conti, A & Lembo, D 2015, 'Identification of equine lactadherin-derived peptides that inhibit rotavirus infection via integrin receptor competition', Journal of Biological Chemistry, vol. 290, no. 19, pp. 12403-12414. https://doi.org/10.1074/jbc.M114.620500
Civra, Andrea ; Giuffrida, Maria Gabriella ; Donalisio, Manuela ; Napolitano, Lorenzo ; Takada, Yoshikazu ; Coulson, Barbara S. ; Conti, Amedeo ; Lembo, David. / Identification of equine lactadherin-derived peptides that inhibit rotavirus infection via integrin receptor competition. In: Journal of Biological Chemistry. 2015 ; Vol. 290, No. 19. pp. 12403-12414.
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