Identification of dried rhizomes of Coptis species using random amplified polymorphic DNA

Kur Ta Cheng, Hsien Chang Chang, Ching Hua Su, Feng Lin Hsu

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The Random amplified polymorphic DNA (RAPD) technique was adopted in the study to identify eight kinds of Coptidis Rhizomes and one kind of Picrorhizae Rhizoma, a pharmaceutical replacement for the former in some provinces of China. The DNA isolated from the dried rhizome of the samples were used as templates in polymerase chain reactions with twenty random decamer primers. Four of the primers, OPT-09, OPT-15, OPT-18, and OPT-20, revealed reproducibly distinct RAPD profiles. The primers possessed different abilities to detect variations in the Coptis species. RAPD analysis provides an efficient approach to identifying the sources of Coptidis Rhizoma medicines on the market.

Original languageEnglish
Pages (from-to)241-244
Number of pages4
JournalBotanical Bulletin of Academia Sinica
Volume38
Issue number4
Publication statusPublished - 1997

Keywords

  • Coptidis
  • Coptis
  • Fingerprinting
  • Identification
  • RAPD

ASJC Scopus subject areas

  • Plant Science

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