Identification of cytokines in whole blood for differential diagnosis of tuberculosis versus pneumonia

Wen Lin Su, Wann Cherng Perng, Ching Hui Huang, Cheng Yu Yang, Chin Pyng Wu, Feng Yee Chang, Jenn Han Chen

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6 Citations (Scopus)

Abstract

Differentiating tuberculosis (TB) from pneumonia remains a challenge. We evaluated the cytokine profiles of whole blood cells from patients with TB (n = 38) or pneumonia (n = 30) and from healthy individuals (n = 30) before and after stimulating cells with ESAT-6 or lipopolysaccharide (LPS). When the percent change in the levels of gamma interferon (IFN-γ) after stimulation with ESAT-6 was used in receiver operating characteristics (ROC) analysis (a graphic method to determine the diagnostic accuracy of a test) to identify a patient with TB, the area under the curve (AUC) was 90.4%, and a cutoff point of a 3.59% change produced a corresponding sensitivity, specificity, and accuracy of over 80%. When the change in IFN-γ after stimulation of blood cells with LPS was used to identify a patient with pneumonia, the AUC reached 89.1%, and a cutoff point of 3.59% produced a sensitivity, specificity, and accuracy of approximately 80% each. When the change in interleukin-12 (IL-12) after stimulation of blood cells with LPS was selected to define a patient with pneumonia, the AUC was 85.2%, and a cutoff point of 2.08% gave a sensitivity, specificity, and accuracy of 80.0%, 78.9%, and 79.4%, respectively. We conclude that the percent change in IFN-γ after stimulation of whole blood cells with ESAT-6 may differentiate patients with TB from patients with pneumonia. The percent change in IFN-γ and IL-12 after LPS stimulation of whole blood cells could differentiate patients with pneumonia from patients with TB.

Original languageEnglish
Pages (from-to)771-777
Number of pages7
JournalClinical and Vaccine Immunology
Volume17
Issue number5
DOIs
Publication statusPublished - May 2010

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ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Su, W. L., Perng, W. C., Huang, C. H., Yang, C. Y., Wu, C. P., Chang, F. Y., & Chen, J. H. (2010). Identification of cytokines in whole blood for differential diagnosis of tuberculosis versus pneumonia. Clinical and Vaccine Immunology, 17(5), 771-777. https://doi.org/10.1128/CVI.00526-09