Identification of baicalin from Bofutsushosan and Daisaikoto as a potent inducer of glucose uptake and modulator of insulin signaling-associated pathways

Yu Ting Kuo, Chih Chan Lin, Hsiao Tzu Kuo, Jui Hsiang Hung, Ching Hsuan Liu, Alagie Jassey, Ming Hong Yen, Shu Jing Wu, Liang Tzung Lin

Research output: Contribution to journalArticle

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Abstract

Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by hyperglycemia that can lead to long-term complications including heart diseases, stroke, retinopathy, and renal failure. Treatment strategies include stimulating glucose uptake and controlling blood glucose level. Bofutsushosan (BOF) and Daisaikoto (DAI) are two herb-based kampo medicines that have been demonstrated to improve metabolism-associated disorders including obesity, hyperlipidemia, and nonalcoholic fatty liver. Given their bioactivities against metabolic syndromes, we explored in this study the effect of BOF and DAI extracts on glucose absorption and used them as source to identify phytochemical stimulator of glucose absorption. Glucose uptake and mechanistic studies were evaluated in differentiated C2C12 skeletal muscle cells, and HPLC analysis was used to determine the molecular bioactive constituents. Our results indicated that the ethanolic extracts of BOF and DAI (BOFEE and DAIEE, respectively) enhanced the glucose uptake ratio in the differentiated C2C12 cells, and further analysis identified the flavone baicalin as a major constituent capable of efficiently stimulating glucose absorption. Mechanistic studies revealed that the effect from baicalin involved the activation of IRS-1 and GLUT-4, and implicated the AMPK, PI3K/Akt, and MAPK/ERK signaling cascades. Due to its potency, we suggest that baicalin merit further evaluation as a potential candidate anti-hyperglycemic agent for the treatment and management of T2DM.

Original languageEnglish
JournalJournal of Food and Drug Analysis
Volume27
DOIs
Publication statusPublished - Jan 2019

Fingerprint

insulin
Insulin
uptake mechanisms
Glucose
glucose
flavone
noninsulin-dependent diabetes mellitus
Type 2 Diabetes Mellitus
Kampo Medicine
hypoglycemic agents
retinal diseases
AMP-Activated Protein Kinases
fatty liver
metabolic syndrome
phosphatidylinositol 3-kinase
extracts
heart diseases
Phytochemicals
hyperlipidemia
renal failure

Keywords

  • Anti-hyperglycemia
  • Baicalin
  • Bofutsushosan
  • Daisaikoto
  • Glucose uptake

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

Identification of baicalin from Bofutsushosan and Daisaikoto as a potent inducer of glucose uptake and modulator of insulin signaling-associated pathways. / Kuo, Yu Ting; Lin, Chih Chan; Kuo, Hsiao Tzu; Hung, Jui Hsiang; Liu, Ching Hsuan; Jassey, Alagie; Yen, Ming Hong; Wu, Shu Jing; Lin, Liang Tzung.

In: Journal of Food and Drug Analysis, Vol. 27, 01.2019.

Research output: Contribution to journalArticle

Kuo, Yu Ting ; Lin, Chih Chan ; Kuo, Hsiao Tzu ; Hung, Jui Hsiang ; Liu, Ching Hsuan ; Jassey, Alagie ; Yen, Ming Hong ; Wu, Shu Jing ; Lin, Liang Tzung. / Identification of baicalin from Bofutsushosan and Daisaikoto as a potent inducer of glucose uptake and modulator of insulin signaling-associated pathways. In: Journal of Food and Drug Analysis. 2019 ; Vol. 27.
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AU - Lin, Chih Chan

AU - Kuo, Hsiao Tzu

AU - Hung, Jui Hsiang

AU - Liu, Ching Hsuan

AU - Jassey, Alagie

AU - Yen, Ming Hong

AU - Wu, Shu Jing

AU - Lin, Liang Tzung

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N2 - Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by hyperglycemia that can lead to long-term complications including heart diseases, stroke, retinopathy, and renal failure. Treatment strategies include stimulating glucose uptake and controlling blood glucose level. Bofutsushosan (BOF) and Daisaikoto (DAI) are two herb-based kampo medicines that have been demonstrated to improve metabolism-associated disorders including obesity, hyperlipidemia, and nonalcoholic fatty liver. Given their bioactivities against metabolic syndromes, we explored in this study the effect of BOF and DAI extracts on glucose absorption and used them as source to identify phytochemical stimulator of glucose absorption. Glucose uptake and mechanistic studies were evaluated in differentiated C2C12 skeletal muscle cells, and HPLC analysis was used to determine the molecular bioactive constituents. Our results indicated that the ethanolic extracts of BOF and DAI (BOFEE and DAIEE, respectively) enhanced the glucose uptake ratio in the differentiated C2C12 cells, and further analysis identified the flavone baicalin as a major constituent capable of efficiently stimulating glucose absorption. Mechanistic studies revealed that the effect from baicalin involved the activation of IRS-1 and GLUT-4, and implicated the AMPK, PI3K/Akt, and MAPK/ERK signaling cascades. Due to its potency, we suggest that baicalin merit further evaluation as a potential candidate anti-hyperglycemic agent for the treatment and management of T2DM.

AB - Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by hyperglycemia that can lead to long-term complications including heart diseases, stroke, retinopathy, and renal failure. Treatment strategies include stimulating glucose uptake and controlling blood glucose level. Bofutsushosan (BOF) and Daisaikoto (DAI) are two herb-based kampo medicines that have been demonstrated to improve metabolism-associated disorders including obesity, hyperlipidemia, and nonalcoholic fatty liver. Given their bioactivities against metabolic syndromes, we explored in this study the effect of BOF and DAI extracts on glucose absorption and used them as source to identify phytochemical stimulator of glucose absorption. Glucose uptake and mechanistic studies were evaluated in differentiated C2C12 skeletal muscle cells, and HPLC analysis was used to determine the molecular bioactive constituents. Our results indicated that the ethanolic extracts of BOF and DAI (BOFEE and DAIEE, respectively) enhanced the glucose uptake ratio in the differentiated C2C12 cells, and further analysis identified the flavone baicalin as a major constituent capable of efficiently stimulating glucose absorption. Mechanistic studies revealed that the effect from baicalin involved the activation of IRS-1 and GLUT-4, and implicated the AMPK, PI3K/Akt, and MAPK/ERK signaling cascades. Due to its potency, we suggest that baicalin merit further evaluation as a potential candidate anti-hyperglycemic agent for the treatment and management of T2DM.

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