Identification of antihypertensive peptides from peptic digest of two microalgae, Chlorella vulgaris and Spirulina platensis

Kunio Suetsuna, Jiun-Rong Chen

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The peptidic fractions that inhibited angiotensin I-converting enzyme (ACE) were separated from the peptic digests of 2 microalgae, Chlorella vulgaris and Spirulina platensis, by ion exchange chromatography and gel filtration. Oral administration of peptidic fractions into spontaneously hypertensive rats at 200 mg/kg of body weight resulted in marked antihypertensive effects. Further separation of the peptidic fractions by ODS high-performance liquid chromatography furnished the following active peptides: Ile-Val-Val-Glu (inhibitory against ACE with an IC50 of 315.3 μM), Ala-Phe-Leu (63.8 μM), Phe-Ala-Leu (26.3 μM), Ala-Glu-Leu (57.1 μM), and Val-Val-Pro-Pro-Ala (79.5 μM) from C. vulgaris; Ile-Ala-Glu (34.7 μM), Phe-Ala-Leu, Ala-Glu-Leu, Ile-Ala-Pro-Gly (11.4 μM), and Val-Ala-Phe (35.8 μM) from S. platensis.

Original languageEnglish
Pages (from-to)305-309
Number of pages5
JournalMarine Biotechnology
Volume3
Issue number4
DOIs
Publication statusPublished - 2001

Fingerprint

Chlorella vulgaris
Spirulina
Microalgae
Spirulina platensis
antihypertensive agents
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A
microalgae
peptide
Antihypertensive Agents
Digestion
digestion
peptides
enzyme
antihypertensive effect
Peptides
Ion Exchange Chromatography
Inbred SHR Rats
ion exchange chromatography
oral administration

Keywords

  • Angiotensin I-converting enzyme
  • Chlorella vulgaris
  • Peptic digest
  • Peptide
  • Spirulina platensis
  • Spontaneously hypertensive rat

ASJC Scopus subject areas

  • Aquatic Science
  • Biotechnology

Cite this

Identification of antihypertensive peptides from peptic digest of two microalgae, Chlorella vulgaris and Spirulina platensis. / Suetsuna, Kunio; Chen, Jiun-Rong.

In: Marine Biotechnology, Vol. 3, No. 4, 2001, p. 305-309.

Research output: Contribution to journalArticle

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