Abstract
The peptidic fractions that inhibited angiotensin I-converting enzyme (ACE) were separated from the peptic digests of 2 microalgae, Chlorella vulgaris and Spirulina platensis, by ion exchange chromatography and gel filtration. Oral administration of peptidic fractions into spontaneously hypertensive rats at 200 mg/kg of body weight resulted in marked antihypertensive effects. Further separation of the peptidic fractions by ODS high-performance liquid chromatography furnished the following active peptides: Ile-Val-Val-Glu (inhibitory against ACE with an IC50 of 315.3 μM), Ala-Phe-Leu (63.8 μM), Phe-Ala-Leu (26.3 μM), Ala-Glu-Leu (57.1 μM), and Val-Val-Pro-Pro-Ala (79.5 μM) from C. vulgaris; Ile-Ala-Glu (34.7 μM), Phe-Ala-Leu, Ala-Glu-Leu, Ile-Ala-Pro-Gly (11.4 μM), and Val-Ala-Phe (35.8 μM) from S. platensis.
| Original language | English |
|---|---|
| Pages (from-to) | 305-309 |
| Number of pages | 5 |
| Journal | Marine Biotechnology |
| Volume | 3 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2001 |
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Keywords
- Angiotensin I-converting enzyme
- Chlorella vulgaris
- Peptic digest
- Peptide
- Spirulina platensis
- Spontaneously hypertensive rat
ASJC Scopus subject areas
- Aquatic Science
- Biotechnology
Cite this
Identification of antihypertensive peptides from peptic digest of two microalgae, Chlorella vulgaris and Spirulina platensis. / Suetsuna, Kunio; Chen, Jiun-Rong.
In: Marine Biotechnology, Vol. 3, No. 4, 2001, p. 305-309.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of antihypertensive peptides from peptic digest of two microalgae, Chlorella vulgaris and Spirulina platensis
AU - Suetsuna, Kunio
AU - Chen, Jiun-Rong
PY - 2001
Y1 - 2001
N2 - The peptidic fractions that inhibited angiotensin I-converting enzyme (ACE) were separated from the peptic digests of 2 microalgae, Chlorella vulgaris and Spirulina platensis, by ion exchange chromatography and gel filtration. Oral administration of peptidic fractions into spontaneously hypertensive rats at 200 mg/kg of body weight resulted in marked antihypertensive effects. Further separation of the peptidic fractions by ODS high-performance liquid chromatography furnished the following active peptides: Ile-Val-Val-Glu (inhibitory against ACE with an IC50 of 315.3 μM), Ala-Phe-Leu (63.8 μM), Phe-Ala-Leu (26.3 μM), Ala-Glu-Leu (57.1 μM), and Val-Val-Pro-Pro-Ala (79.5 μM) from C. vulgaris; Ile-Ala-Glu (34.7 μM), Phe-Ala-Leu, Ala-Glu-Leu, Ile-Ala-Pro-Gly (11.4 μM), and Val-Ala-Phe (35.8 μM) from S. platensis.
AB - The peptidic fractions that inhibited angiotensin I-converting enzyme (ACE) were separated from the peptic digests of 2 microalgae, Chlorella vulgaris and Spirulina platensis, by ion exchange chromatography and gel filtration. Oral administration of peptidic fractions into spontaneously hypertensive rats at 200 mg/kg of body weight resulted in marked antihypertensive effects. Further separation of the peptidic fractions by ODS high-performance liquid chromatography furnished the following active peptides: Ile-Val-Val-Glu (inhibitory against ACE with an IC50 of 315.3 μM), Ala-Phe-Leu (63.8 μM), Phe-Ala-Leu (26.3 μM), Ala-Glu-Leu (57.1 μM), and Val-Val-Pro-Pro-Ala (79.5 μM) from C. vulgaris; Ile-Ala-Glu (34.7 μM), Phe-Ala-Leu, Ala-Glu-Leu, Ile-Ala-Pro-Gly (11.4 μM), and Val-Ala-Phe (35.8 μM) from S. platensis.
KW - Angiotensin I-converting enzyme
KW - Chlorella vulgaris
KW - Peptic digest
KW - Peptide
KW - Spirulina platensis
KW - Spontaneously hypertensive rat
UR - http://www.scopus.com/inward/record.url?scp=0034817211&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034817211&partnerID=8YFLogxK
U2 - 10.1007/s10126-001-0012-7
DO - 10.1007/s10126-001-0012-7
M3 - Article
C2 - 14961345
AN - SCOPUS:0034817211
VL - 3
SP - 305
EP - 309
JO - Marine Biotechnology
JF - Marine Biotechnology
SN - 1436-2228
IS - 4
ER -