Identification of a novel cephalosporinase (DHA-3) in Klebsiella pneumoniae isolated in Taiwan

L. T. Wu, S. W. Hung, Y. C. Chuang, H. E. Chen, R. N. Jones, W. Leon Yu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A strain of Klebsiella pneumoniae resistant to cefoxitin and oxyimino-cephalosporins, but susceptible to cefepime, was isolated from an adult patient hospitalised in Taichung, Taiwan. Isoelectric focusing revealed three β-lactamases with isoelectric points of 5.4, 8.2 and 7.9, respectively. Following PCR with plasmid DNA templates and gene sequencing, these enzymes were shown to correspond to TEM-1, SHV-5 and a novel DHA-1-like enzyme (designated DHA-3). The bla genes for TEM-1 and SHV-5 were transferable, but the bla DHA-3 gene was non-self-transferable in conjugation experiments. All three bla genes were successfully introduced by electrotransformation into an Escherichia coli recipient (DH5α), resulting in a similar resistance profile to that observed in the original donor strain. Other K. pneumoniae strains producing DHA-1-like enzymes have been identified previously in Taiwan, and this report suggests that DHA-type β-lactamases are continuing to emerge in this country.

Original languageEnglish
Pages (from-to)893-897
Number of pages5
JournalClinical Microbiology and Infection
Volume11
Issue number11
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Cephalosporinase
Klebsiella pneumoniae
Taiwan
Genes
Enzymes
Cefoxitin
Isoelectric Point
Isoelectric Focusing
Cephalosporins
Plasmids
Escherichia coli
Polymerase Chain Reaction
DNA

Keywords

  • β-lactamases
  • Antibiotic resistance
  • DHA-type β-lactamases
  • Klebsiella pneumoniae
  • Resistance
  • Taiwan

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Identification of a novel cephalosporinase (DHA-3) in Klebsiella pneumoniae isolated in Taiwan. / Wu, L. T.; Hung, S. W.; Chuang, Y. C.; Chen, H. E.; Jones, R. N.; Yu, W. Leon.

In: Clinical Microbiology and Infection, Vol. 11, No. 11, 11.2005, p. 893-897.

Research output: Contribution to journalArticle

Wu, L. T. ; Hung, S. W. ; Chuang, Y. C. ; Chen, H. E. ; Jones, R. N. ; Yu, W. Leon. / Identification of a novel cephalosporinase (DHA-3) in Klebsiella pneumoniae isolated in Taiwan. In: Clinical Microbiology and Infection. 2005 ; Vol. 11, No. 11. pp. 893-897.
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