Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma

Ann Ping Tsou, Chu Wen Yang, Chi Ying F Huang, Ricky Chang Tze Yu, Yuan Chii G Lee, Cha Wei Chang, Bo Rue Chen, Yu Fang Chung, Ming Ji Fann, Chin Wen Chi, Jen Hwey Chiu, Chen Kung Chou

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

An analytic strategy was followed to identify putative regulatory genes during the development of human hepatocellular carcinoma (HCC). This strategy employed a bioinformatics analysis that used a database search to identify genes, which are differentially expressed in human HCC and are also under cell cycle regulation. A novel cell cycle regulated gene (HURP) that is overexpressed in HCC was identified. Full-length cDNAs encoding the human and mouse HURP genes were isolated. They share 72 and 61% identity at the nucleotide level and amino-acid level, respectively. Endogenous levels of HURP mRNA were found to be tightly regulated during cell cycle progression as illustrated by its elevated expression in the G2/M phase of synchronized HeLa cells and in regenerating mouse liver after partial hepatectomy. Immunofluorescence studies revealed that hepatoma up-regulated protein (HURP) localizes to the spindle poles during mitosis. Overexpression of HURP in 293T cells resulted in an enhanced cell growth at low serum levels and at polyhema-based, anchorage-independent growth assay. Taken together, these results strongly suggest that HURP is a potential novel cell cycle regulator that may play a role in the carcinogenesis of human cancer cells.

Original languageEnglish
Pages (from-to)298-307
Number of pages10
JournalOncogene
Volume22
Issue number2
DOIs
Publication statusPublished - Jan 16 2003
Externally publishedYes

Fingerprint

cdc Genes
Hepatocellular Carcinoma
Proteins
Cell Cycle
Spindle Poles
HEK293 Cells
G2 Phase
Human Development
Hepatectomy
Regulator Genes
Growth
Computational Biology
HeLa Cells
Mitosis
Cell Division
Genes
Fluorescent Antibody Technique
Carcinogenesis
Nucleotides
Complementary DNA

Keywords

  • Bioinformatics
  • Cell cycle regulator
  • Heptocellular carcinoma
  • HURP
  • Liver regeneration

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Tsou, A. P., Yang, C. W., Huang, C. Y. F., Yu, R. C. T., Lee, Y. C. G., Chang, C. W., ... Chou, C. K. (2003). Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma. Oncogene, 22(2), 298-307. https://doi.org/10.1038/sj.onc.1206129

Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma. / Tsou, Ann Ping; Yang, Chu Wen; Huang, Chi Ying F; Yu, Ricky Chang Tze; Lee, Yuan Chii G; Chang, Cha Wei; Chen, Bo Rue; Chung, Yu Fang; Fann, Ming Ji; Chi, Chin Wen; Chiu, Jen Hwey; Chou, Chen Kung.

In: Oncogene, Vol. 22, No. 2, 16.01.2003, p. 298-307.

Research output: Contribution to journalArticle

Tsou, AP, Yang, CW, Huang, CYF, Yu, RCT, Lee, YCG, Chang, CW, Chen, BR, Chung, YF, Fann, MJ, Chi, CW, Chiu, JH & Chou, CK 2003, 'Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma', Oncogene, vol. 22, no. 2, pp. 298-307. https://doi.org/10.1038/sj.onc.1206129
Tsou, Ann Ping ; Yang, Chu Wen ; Huang, Chi Ying F ; Yu, Ricky Chang Tze ; Lee, Yuan Chii G ; Chang, Cha Wei ; Chen, Bo Rue ; Chung, Yu Fang ; Fann, Ming Ji ; Chi, Chin Wen ; Chiu, Jen Hwey ; Chou, Chen Kung. / Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma. In: Oncogene. 2003 ; Vol. 22, No. 2. pp. 298-307.
@article{31dc02d5df3c4a1d9d76437861d9a415,
title = "Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma",
abstract = "An analytic strategy was followed to identify putative regulatory genes during the development of human hepatocellular carcinoma (HCC). This strategy employed a bioinformatics analysis that used a database search to identify genes, which are differentially expressed in human HCC and are also under cell cycle regulation. A novel cell cycle regulated gene (HURP) that is overexpressed in HCC was identified. Full-length cDNAs encoding the human and mouse HURP genes were isolated. They share 72 and 61{\%} identity at the nucleotide level and amino-acid level, respectively. Endogenous levels of HURP mRNA were found to be tightly regulated during cell cycle progression as illustrated by its elevated expression in the G2/M phase of synchronized HeLa cells and in regenerating mouse liver after partial hepatectomy. Immunofluorescence studies revealed that hepatoma up-regulated protein (HURP) localizes to the spindle poles during mitosis. Overexpression of HURP in 293T cells resulted in an enhanced cell growth at low serum levels and at polyhema-based, anchorage-independent growth assay. Taken together, these results strongly suggest that HURP is a potential novel cell cycle regulator that may play a role in the carcinogenesis of human cancer cells.",
keywords = "Bioinformatics, Cell cycle regulator, Heptocellular carcinoma, HURP, Liver regeneration",
author = "Tsou, {Ann Ping} and Yang, {Chu Wen} and Huang, {Chi Ying F} and Yu, {Ricky Chang Tze} and Lee, {Yuan Chii G} and Chang, {Cha Wei} and Chen, {Bo Rue} and Chung, {Yu Fang} and Fann, {Ming Ji} and Chi, {Chin Wen} and Chiu, {Jen Hwey} and Chou, {Chen Kung}",
year = "2003",
month = "1",
day = "16",
doi = "10.1038/sj.onc.1206129",
language = "English",
volume = "22",
pages = "298--307",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma

AU - Tsou, Ann Ping

AU - Yang, Chu Wen

AU - Huang, Chi Ying F

AU - Yu, Ricky Chang Tze

AU - Lee, Yuan Chii G

AU - Chang, Cha Wei

AU - Chen, Bo Rue

AU - Chung, Yu Fang

AU - Fann, Ming Ji

AU - Chi, Chin Wen

AU - Chiu, Jen Hwey

AU - Chou, Chen Kung

PY - 2003/1/16

Y1 - 2003/1/16

N2 - An analytic strategy was followed to identify putative regulatory genes during the development of human hepatocellular carcinoma (HCC). This strategy employed a bioinformatics analysis that used a database search to identify genes, which are differentially expressed in human HCC and are also under cell cycle regulation. A novel cell cycle regulated gene (HURP) that is overexpressed in HCC was identified. Full-length cDNAs encoding the human and mouse HURP genes were isolated. They share 72 and 61% identity at the nucleotide level and amino-acid level, respectively. Endogenous levels of HURP mRNA were found to be tightly regulated during cell cycle progression as illustrated by its elevated expression in the G2/M phase of synchronized HeLa cells and in regenerating mouse liver after partial hepatectomy. Immunofluorescence studies revealed that hepatoma up-regulated protein (HURP) localizes to the spindle poles during mitosis. Overexpression of HURP in 293T cells resulted in an enhanced cell growth at low serum levels and at polyhema-based, anchorage-independent growth assay. Taken together, these results strongly suggest that HURP is a potential novel cell cycle regulator that may play a role in the carcinogenesis of human cancer cells.

AB - An analytic strategy was followed to identify putative regulatory genes during the development of human hepatocellular carcinoma (HCC). This strategy employed a bioinformatics analysis that used a database search to identify genes, which are differentially expressed in human HCC and are also under cell cycle regulation. A novel cell cycle regulated gene (HURP) that is overexpressed in HCC was identified. Full-length cDNAs encoding the human and mouse HURP genes were isolated. They share 72 and 61% identity at the nucleotide level and amino-acid level, respectively. Endogenous levels of HURP mRNA were found to be tightly regulated during cell cycle progression as illustrated by its elevated expression in the G2/M phase of synchronized HeLa cells and in regenerating mouse liver after partial hepatectomy. Immunofluorescence studies revealed that hepatoma up-regulated protein (HURP) localizes to the spindle poles during mitosis. Overexpression of HURP in 293T cells resulted in an enhanced cell growth at low serum levels and at polyhema-based, anchorage-independent growth assay. Taken together, these results strongly suggest that HURP is a potential novel cell cycle regulator that may play a role in the carcinogenesis of human cancer cells.

KW - Bioinformatics

KW - Cell cycle regulator

KW - Heptocellular carcinoma

KW - HURP

KW - Liver regeneration

UR - http://www.scopus.com/inward/record.url?scp=0037448686&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037448686&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1206129

DO - 10.1038/sj.onc.1206129

M3 - Article

C2 - 12527899

AN - SCOPUS:0037448686

VL - 22

SP - 298

EP - 307

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 2

ER -