Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma

Ann Ping Tsou, Chu Wen Yang, Chi Ying F Huang, Ricky Chang Tze Yu, Yuan Chii G Lee, Cha Wei Chang, Bo Rue Chen, Yu Fang Chung, Ming Ji Fann, Chin Wen Chi, Jen Hwey Chiu, Chen Kung Chou

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

An analytic strategy was followed to identify putative regulatory genes during the development of human hepatocellular carcinoma (HCC). This strategy employed a bioinformatics analysis that used a database search to identify genes, which are differentially expressed in human HCC and are also under cell cycle regulation. A novel cell cycle regulated gene (HURP) that is overexpressed in HCC was identified. Full-length cDNAs encoding the human and mouse HURP genes were isolated. They share 72 and 61% identity at the nucleotide level and amino-acid level, respectively. Endogenous levels of HURP mRNA were found to be tightly regulated during cell cycle progression as illustrated by its elevated expression in the G2/M phase of synchronized HeLa cells and in regenerating mouse liver after partial hepatectomy. Immunofluorescence studies revealed that hepatoma up-regulated protein (HURP) localizes to the spindle poles during mitosis. Overexpression of HURP in 293T cells resulted in an enhanced cell growth at low serum levels and at polyhema-based, anchorage-independent growth assay. Taken together, these results strongly suggest that HURP is a potential novel cell cycle regulator that may play a role in the carcinogenesis of human cancer cells.

Original languageEnglish
Pages (from-to)298-307
Number of pages10
JournalOncogene
Volume22
Issue number2
DOIs
Publication statusPublished - Jan 16 2003
Externally publishedYes

Keywords

  • Bioinformatics
  • Cell cycle regulator
  • Heptocellular carcinoma
  • HURP
  • Liver regeneration

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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