Identification of a lipoxygenase inhibitor in A431 cells as a phospholipid hydroperoxide glutathione peroxidase

Hei Sheng Huang, Ching Jiunn Chen, Hsieng S. Lu, Wen Chang Chang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

An endogenous lipoxygenase inhibitor, purified from the cytosol of human epidermoid carcinoma A431 cells, was analyzed by N-terminal microsequencing and mass spectrometric analysis. The inhibitor was purified by SDS-PAGE, then subjected to in-gel CNBr cleavage and trypsin digestion. The N-terminal sequence data obtained from a 6-8 kDa band of in-gel CNBr cleavage and the three isolated peptides of in-gel trypsin digestion, and the C-terminal peptide sequence from matrix-assisted laser desorption ionization mass spectrometry matched the sequence of human phospholipid hydroperoxide glutathione peroxidase. The purified inhibitor exhibited peroxidase activity using phosphatidylcholine hydroperoxides as the substrate. We therefore concluded that the lipoxygenase inhibitor present in A431 cells was a phospholipid hydroperoxide glutathione peroxidase.

Original languageEnglish
Pages (from-to)22-26
Number of pages5
JournalFEBS Letters
Volume424
Issue number1-2
DOIs
Publication statusPublished - Mar 6 1998
Externally publishedYes

Fingerprint

phospholipid-hydroperoxide glutathione peroxidase
Lipoxygenase Inhibitors
Gels
Trypsin
Digestion
Peptides
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Cytosol
Peroxidase
Ionization
Mass spectrometry
Polyacrylamide Gel Electrophoresis
Squamous Cell Carcinoma
Desorption
Lasers
Substrates

Keywords

  • A431 cell
  • Lipoxygenase inhibitor
  • Microsequencing
  • PHGPx

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of a lipoxygenase inhibitor in A431 cells as a phospholipid hydroperoxide glutathione peroxidase. / Huang, Hei Sheng; Chen, Ching Jiunn; Lu, Hsieng S.; Chang, Wen Chang.

In: FEBS Letters, Vol. 424, No. 1-2, 06.03.1998, p. 22-26.

Research output: Contribution to journalArticle

Huang, Hei Sheng ; Chen, Ching Jiunn ; Lu, Hsieng S. ; Chang, Wen Chang. / Identification of a lipoxygenase inhibitor in A431 cells as a phospholipid hydroperoxide glutathione peroxidase. In: FEBS Letters. 1998 ; Vol. 424, No. 1-2. pp. 22-26.
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