Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro

James Wang, Xiaoyu Guo, Monica Lui, Pei Ju Chu, Jennifer Yoo, Megan Chang, Yun Yen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Small stem cells, such as spore-like cells, blastomere-like stem cells (BLSCs), and very-small embryonic-like stem cells (VSELs) have been described in recent studies, although their multipotency in human tissues has not yet been confirmed. Here, we report the discovery of adult multipotent stem cells derived from human bone marrow, which we call StemBios (SB) cells. These isolated SB cells are smaller than 6 ìm and are DAPI+ and Lgr5 + (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5). Because Lgr5 has been characterized as a stem cell marker in the intestine, we hypothesized that SB cells may have a similar function. In vivo cell tracking assays confirmed that SB cells give rise to three types of cells, and in vitro studies demonstrated that SB cells cultured in proprietary media are able to grow to 6-25 ìm in size. Once the SB cells have attached to the wells, they differentiate into different cell lineages upon exposure to specific differentiation media. We are the first to demonstrate that stem cells smaller than 6 ìm can differentiate both in vivo and in vitro. In the future, we hope that SB cells will be used therapeutically to cure degenerative diseases.

Original languageEnglish
Article numbere85112
JournalPLoS One
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 17 2014
Externally publishedYes

Fingerprint

Stem cells
bone marrow
Bone
Bone Marrow
Cells
Population
stem cells
Stem Cells
cells
Cell Tracking
Multipotent Stem Cells
G-Protein-Coupled Receptors
Leucine
Blastomeres
Adult Stem Cells
In Vitro Techniques
Assays
Cell Lineage
Embryonic Stem Cells
Spores

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro. / Wang, James; Guo, Xiaoyu; Lui, Monica; Chu, Pei Ju; Yoo, Jennifer; Chang, Megan; Yen, Yun.

In: PLoS One, Vol. 9, No. 1, e85112, 17.01.2014.

Research output: Contribution to journalArticle

Wang, James ; Guo, Xiaoyu ; Lui, Monica ; Chu, Pei Ju ; Yoo, Jennifer ; Chang, Megan ; Yen, Yun. / Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro. In: PLoS One. 2014 ; Vol. 9, No. 1.
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