Identification and functional analysis of an ovarian form of the egg activation factor phospholipase C zeta (PLCζ) in pufferfish

Kevin Coward, Chris P. Ponting, Nan Zhang, Claire Young, Chang Jen Huang, Chih Ming Chou, Junaid Kashir, Rafael A. Fissore, John Parrington

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Recent studies suggest that egg activation in mammals is triggered by a sperm-specific phospholipase C, PLCzeta. In other vertebrate species such as medaka fish, chickens, and quail, PLCzeta is also expressed as a testis-specific mRNA. Functional studies suggest that PLCzeta plays a similar role as a trigger of egg activation in these species. Here, we report the identification of PLCzeta orthologues in pufferfish species Takifugu rubripes (Fugu) and Tetraodon nigroviridis (Tetraodon). Unexpectedly in these species PLCzeta is expressed not in the testis, but in ovary and brain. Injection of pufferfish PLCzeta copy ribonucleic acid (cRNA) into mouse eggs failed to trigger calcium oscillations, unlike medaka PLCzeta cRNA. Our findings provide the first evidence that PLCzeta may be expressed in the egg, rather than the sperm, in some vertebrate species, and that its mechanism of action and physiologic role at fertilization may differ in different vertebrate species.

Original languageEnglish
Pages (from-to)48-56
Number of pages9
JournalMolecular Reproduction and Development
Volume78
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Tetraodontiformes
Type C Phospholipases
Takifugu
Oryzias
Ovum
Vertebrates
Spermatozoa
Testis
RNA
Quail
Calcium Signaling
Fertilization
Eggs
Mammals
Ovary
Chickens
Fishes
Messenger RNA
Injections
Brain

Keywords

  • Calcium
  • Egg activation
  • Fertilization
  • Phospholipase C zeta
  • Pufferfish

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Identification and functional analysis of an ovarian form of the egg activation factor phospholipase C zeta (PLCζ) in pufferfish. / Coward, Kevin; Ponting, Chris P.; Zhang, Nan; Young, Claire; Huang, Chang Jen; Chou, Chih Ming; Kashir, Junaid; Fissore, Rafael A.; Parrington, John.

In: Molecular Reproduction and Development, Vol. 78, No. 1, 01.2011, p. 48-56.

Research output: Contribution to journalArticle

Coward, Kevin ; Ponting, Chris P. ; Zhang, Nan ; Young, Claire ; Huang, Chang Jen ; Chou, Chih Ming ; Kashir, Junaid ; Fissore, Rafael A. ; Parrington, John. / Identification and functional analysis of an ovarian form of the egg activation factor phospholipase C zeta (PLCζ) in pufferfish. In: Molecular Reproduction and Development. 2011 ; Vol. 78, No. 1. pp. 48-56.
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