Identification and characterization of a hyperglycemic hormone from freshwater giant prawn, Macrobrachium rosenbergii

C. Y. Lin, S. H. Chen, G. H. Kou, C. M. Kuo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Crustacean hyperglycemic hormone (CHH), a physiologically important neurohormone stored in the sinus gland of eyestalks, primarily regulates carbohydrate metabolism and also plays significant roles in reproduction, molting and other physiological processes. In the freshwater giant prawn, Macrobrachium rosenbergii, an injection of X-organ sinus gland (XOSG) extract evoked a hyperglycemic response, peaked in 1 h. The hyperglycemic effect of the eyestalk extract was maximal at the dose of 0.5 eyestalk equivalent. CHH fractionated by RP-HPLC, in M. rosenbergii was identified by its hyperglycemic activity and partial amino acid sequence, and the molecular weight of 8534 was determined by matrix-assisted laser desorption ionization mass spectrometry-time of flight analysis (MALDI-TOF). The amino acid sequence of the first 25 residues of CHH showed 72% homology with the first 25 residues of CHH A and CHH B of the American lobster Homarus americanus.

Original languageEnglish
Pages (from-to)315-321
Number of pages7
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume121
Issue number4
DOIs
Publication statusPublished - Dec 1 1998
Externally publishedYes

Keywords

  • CHH
  • Crustaceans
  • Freshwater giant prawns
  • Hyperglycemia
  • Neuropeptide
  • Sinus gland
  • X-organ

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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