Hypoxia and reoxygenation modulate the arrhythmogenic activity of the pulmonary vein and atrium

Yung Kuo Lin, Mei Shou Lai, Yao Chang Chen, Chen Chuan Cheng, Jen Hung Huang, Shih Ann Chen, Yi Jen Chen, Cheng I. Lin

Research output: Contribution to journalArticle

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Abstract

Ischaemia and reperfusion contribute to the genesis of AF (atrial fibrillation). PVs (pulmonary veins) and the atria are important foci for AF initiation and maintenance. However, the effect of ischaemia and reperfusion on PVs and the atria has not yet been fully elucidated. In the present study, conventional microelectrodes were used to record the APs (action potentials) in isolated rabbit PV, LA (left atrium) and RA (right atrium) specimens during hypoxia and reoxygenation, and pharmacological interventions. Hypoxia reduced the PV beating rates from 1.8 ± 0.1 to 1.3 ± 0.2 and 0.8 ± 0.1 Hz at 30 and 60 min respectively (n=8, P <0.005), and induced EAD (early after depolarization) in three (37.5%) of the PVs and DAD (delayed after depolarization) in one (12.5%) of the PVs. Reoxygenation increased the PV spontaneous rate to 1.4 ± 0.2 Hz (P <0.05) and induced PV burst firings (3.5 ± 0.1 Hz, P <0.001) in six (75 %) of the PVs. Hypoxia shortened the AP duration in the LA and PVs, but not in the RA. Pretreatment with glibenclamide attenuated hypoxia-induced decreases in the PV spontaneous activity and the shortening of the LA and PV AP duration. Similar to those in hypoxia, the K ATP (ATP-sensitive potassium) channel opener pinacidil (30μM) decreased PV spontaneous activity and shortened the AP duration. Pretreatment with 5 mM N-MPG [N-(mercaptopropionyl)glycine; a hydroxyl ( OH) free-radical scavenger] or 300μM chloramphenicol [a cytochrome P450 inhibitor that reduces ROS (reactive oxygen species)] attenuated the rate changes induced by hypoxia and reoxygenation, and also decreased the burst firing incidence. In conclusion, hypoxia and reoxygenation significantly increased PV arrhythmogenesis and induced different electrophysiological responses in the RA and LA, which may play a role in the pathophysiology of AF.

Original languageEnglish
Pages (from-to)121-132
Number of pages12
JournalClinical Science
Volume122
Issue number3
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Pulmonary Veins
Heart Atria
Action Potentials
Atrial Fibrillation
Hypoxia
Reperfusion
Ischemia
Pinacidil
KATP Channels
Free Radical Scavengers
Glyburide
Microelectrodes
Chloramphenicol
Hydroxyl Radical
Glycine
Cytochrome P-450 Enzyme System
Reactive Oxygen Species

Keywords

  • Atrial fibrillation (AF)
  • Early after depolarization (EAD)
  • Hypoxia
  • Reoxygenation
  • Resting membrane potential (RMP)

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hypoxia and reoxygenation modulate the arrhythmogenic activity of the pulmonary vein and atrium. / Lin, Yung Kuo; Lai, Mei Shou; Chen, Yao Chang; Cheng, Chen Chuan; Huang, Jen Hung; Chen, Shih Ann; Chen, Yi Jen; Lin, Cheng I.

In: Clinical Science, Vol. 122, No. 3, 02.2012, p. 121-132.

Research output: Contribution to journalArticle

Lin, Yung Kuo ; Lai, Mei Shou ; Chen, Yao Chang ; Cheng, Chen Chuan ; Huang, Jen Hung ; Chen, Shih Ann ; Chen, Yi Jen ; Lin, Cheng I. / Hypoxia and reoxygenation modulate the arrhythmogenic activity of the pulmonary vein and atrium. In: Clinical Science. 2012 ; Vol. 122, No. 3. pp. 121-132.
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