Hypoxia and metabolic phenotypes during breast carcinogenesis: Expression of HIF-1α, GLUT1, and CAIX

Chi Long Chen, Jan Show Chu, Wu Chou Su, Soon Cen Huang, Wen Ying Lee

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Hypoxia and acidosis are microenvironmental selection forces during somatic evolution in breast carcinogenesis. The effect of cobalt chloride (CoCl 2)-induced hypoxia on the expression of hypoxia-inducible factor (HIF)-1α, glucose transporter 1 (GLUT1), and carbonic anhydrase IX (CAIX) was assessed in breast cancer cells derived from primary sites (HCC1395 and HCC1937) and metastatic sites (MCF-7 and MDA-MB-231) by reverse transcriptase-polymerase chain reaction and immunoblotting. We analyzed these proteins' expression in tissue samples from normal breast tissue, usual ductal hyperplasia (DH), atypical ductal hyperplasia (ADH), ductal carcinoma in situ (DCIS), and invasive ductal carcinoma (IDC) using immunohistochemistry. CAIX mRNA was expressed constitutively in MDA-MB-231 cells but not in the other three cell lines. CAIX mRNA expression was increased after CoCl2-induced hypoxia in all four breast cancer cell lines. The expression of HIF-1α and GLUT1 proteins was increased after CoCl2-induced hypoxia in all breast cancer cell lines tested. Hypoxia significantly increased CAIX protein expression in primary cancer cells but not in metastatic ones. HIF-1α was not expressed in benign breast tissue, whereas it was significantly expressed in DH, ADH, DCIS, and IDC (p

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalVirchows Archiv
Volume457
Issue number1
DOIs
Publication statusPublished - Jul 2010

Keywords

  • Breast
  • CAIX
  • Carcinogenesis
  • GLUT1
  • Hypoxia

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology

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