Hypodense alveolar macrophages in patients with diabetes mellitus and active pulmonary tuberculosis

C. H. Wang, C. T. Yu, H. C. Lin, C. Y. Liu, H. P. Kuo

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Setting: Alveolar macrophages (AM), a heterogeneous cell population, play a critical role in eliminating mycobacterial infections in collaboration with lymphocytes. Patients with diabetes mellitus (DM) show increased susceptibility to pulmonary tuberculosis (TB) infection. It is still uncertain whether there is a defect in T cell or AM activation in patients with DM against TB infection. Objective: To study the difference in activation status of AM and T cells between patients with TB+DM and TB alone. Method: The heterogeneity of AM from 14 patients with TB+DM, 9 with TB alone, 10 normal subjects and 8 DM alone patients, was studied using Percoll density fractionation. The intracellular H2O2 production of AM before and after stimulation with phorbol myristate acetate (PMA) or F-Met-Leu-Phen (FMLP) was assayed by loading cells with 2',7'-dichlorofluorescin (DCFH) and analyzed by flow cytometry. Lymphocytes subsets (CD3, CD4, CD8) and their activation status (CD25) in bronchoalveolar lavage were also measured. Results: The proportion of the least dense AM (< 1.030 g/ml) and the magnitude of DCFH oxidation of AM was higher in TB patients than in normal subjects, regardless of DM. Patients with TB+DM had a significantly lower proportion of the least density AM fraction than TB alone patients, regardless of disease extent. Among TB patients, the proportion of the least dense AM was inversely correlated with the bacterial load on sputum and the disease extent on chest radiograph. Stimulation of AM with PMA or FMLP induced an increase in the hypodense AM subpopulations and enhanced intracellular H2O2 generation in patients with TB+DM and to a similar extent in normal subjects, but not in patients with TB alone. There was no significant difference in CD3 numbers, CD4/CD8 ratio, and CD25+ cells between patients with TB alone and TB+DM. The activation status of AM or T lymphocytes from DM alone patients was not significantly different from those from normal subjects. Conclusion: Hypodense subpopulations of AM increase in active TB patients and are related to the disease severity as well as activation status of AM. AM in TB patients complicated with DM was less activated, and may be contributory to the susceptibility to mycobacterial infection.

Original languageEnglish
Pages (from-to)235-242
Number of pages8
JournalTubercle and Lung Disease
Volume79
Issue number4
DOIs
Publication statusPublished - Jan 1 1999
Externally publishedYes

Fingerprint

Alveolar Macrophages
Pulmonary Tuberculosis
Diabetes Mellitus
Tuberculosis
Tetradecanoylphorbol Acetate
Infection
T-Lymphocytes
Alveolar Epithelial Cells
CD4-CD8 Ratio
Macrophage Activation
Bacterial Load
Lymphocyte Subsets
Bronchoalveolar Lavage
Sputum
Flow Cytometry

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

Hypodense alveolar macrophages in patients with diabetes mellitus and active pulmonary tuberculosis. / Wang, C. H.; Yu, C. T.; Lin, H. C.; Liu, C. Y.; Kuo, H. P.

In: Tubercle and Lung Disease, Vol. 79, No. 4, 01.01.1999, p. 235-242.

Research output: Contribution to journalArticle

Wang, C. H. ; Yu, C. T. ; Lin, H. C. ; Liu, C. Y. ; Kuo, H. P. / Hypodense alveolar macrophages in patients with diabetes mellitus and active pulmonary tuberculosis. In: Tubercle and Lung Disease. 1999 ; Vol. 79, No. 4. pp. 235-242.
@article{ebf7bdfb962842c9a3e7835c5e0c618c,
title = "Hypodense alveolar macrophages in patients with diabetes mellitus and active pulmonary tuberculosis",
abstract = "Setting: Alveolar macrophages (AM), a heterogeneous cell population, play a critical role in eliminating mycobacterial infections in collaboration with lymphocytes. Patients with diabetes mellitus (DM) show increased susceptibility to pulmonary tuberculosis (TB) infection. It is still uncertain whether there is a defect in T cell or AM activation in patients with DM against TB infection. Objective: To study the difference in activation status of AM and T cells between patients with TB+DM and TB alone. Method: The heterogeneity of AM from 14 patients with TB+DM, 9 with TB alone, 10 normal subjects and 8 DM alone patients, was studied using Percoll density fractionation. The intracellular H2O2 production of AM before and after stimulation with phorbol myristate acetate (PMA) or F-Met-Leu-Phen (FMLP) was assayed by loading cells with 2',7'-dichlorofluorescin (DCFH) and analyzed by flow cytometry. Lymphocytes subsets (CD3, CD4, CD8) and their activation status (CD25) in bronchoalveolar lavage were also measured. Results: The proportion of the least dense AM (< 1.030 g/ml) and the magnitude of DCFH oxidation of AM was higher in TB patients than in normal subjects, regardless of DM. Patients with TB+DM had a significantly lower proportion of the least density AM fraction than TB alone patients, regardless of disease extent. Among TB patients, the proportion of the least dense AM was inversely correlated with the bacterial load on sputum and the disease extent on chest radiograph. Stimulation of AM with PMA or FMLP induced an increase in the hypodense AM subpopulations and enhanced intracellular H2O2 generation in patients with TB+DM and to a similar extent in normal subjects, but not in patients with TB alone. There was no significant difference in CD3 numbers, CD4/CD8 ratio, and CD25+ cells between patients with TB alone and TB+DM. The activation status of AM or T lymphocytes from DM alone patients was not significantly different from those from normal subjects. Conclusion: Hypodense subpopulations of AM increase in active TB patients and are related to the disease severity as well as activation status of AM. AM in TB patients complicated with DM was less activated, and may be contributory to the susceptibility to mycobacterial infection.",
author = "Wang, {C. H.} and Yu, {C. T.} and Lin, {H. C.} and Liu, {C. Y.} and Kuo, {H. P.}",
year = "1999",
month = "1",
day = "1",
doi = "10.1054/tuld.1998.0167",
language = "English",
volume = "79",
pages = "235--242",
journal = "Tuberculosis",
issn = "1472-9792",
publisher = "Churchill Livingstone",
number = "4",

}

TY - JOUR

T1 - Hypodense alveolar macrophages in patients with diabetes mellitus and active pulmonary tuberculosis

AU - Wang, C. H.

AU - Yu, C. T.

AU - Lin, H. C.

AU - Liu, C. Y.

AU - Kuo, H. P.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Setting: Alveolar macrophages (AM), a heterogeneous cell population, play a critical role in eliminating mycobacterial infections in collaboration with lymphocytes. Patients with diabetes mellitus (DM) show increased susceptibility to pulmonary tuberculosis (TB) infection. It is still uncertain whether there is a defect in T cell or AM activation in patients with DM against TB infection. Objective: To study the difference in activation status of AM and T cells between patients with TB+DM and TB alone. Method: The heterogeneity of AM from 14 patients with TB+DM, 9 with TB alone, 10 normal subjects and 8 DM alone patients, was studied using Percoll density fractionation. The intracellular H2O2 production of AM before and after stimulation with phorbol myristate acetate (PMA) or F-Met-Leu-Phen (FMLP) was assayed by loading cells with 2',7'-dichlorofluorescin (DCFH) and analyzed by flow cytometry. Lymphocytes subsets (CD3, CD4, CD8) and their activation status (CD25) in bronchoalveolar lavage were also measured. Results: The proportion of the least dense AM (< 1.030 g/ml) and the magnitude of DCFH oxidation of AM was higher in TB patients than in normal subjects, regardless of DM. Patients with TB+DM had a significantly lower proportion of the least density AM fraction than TB alone patients, regardless of disease extent. Among TB patients, the proportion of the least dense AM was inversely correlated with the bacterial load on sputum and the disease extent on chest radiograph. Stimulation of AM with PMA or FMLP induced an increase in the hypodense AM subpopulations and enhanced intracellular H2O2 generation in patients with TB+DM and to a similar extent in normal subjects, but not in patients with TB alone. There was no significant difference in CD3 numbers, CD4/CD8 ratio, and CD25+ cells between patients with TB alone and TB+DM. The activation status of AM or T lymphocytes from DM alone patients was not significantly different from those from normal subjects. Conclusion: Hypodense subpopulations of AM increase in active TB patients and are related to the disease severity as well as activation status of AM. AM in TB patients complicated with DM was less activated, and may be contributory to the susceptibility to mycobacterial infection.

AB - Setting: Alveolar macrophages (AM), a heterogeneous cell population, play a critical role in eliminating mycobacterial infections in collaboration with lymphocytes. Patients with diabetes mellitus (DM) show increased susceptibility to pulmonary tuberculosis (TB) infection. It is still uncertain whether there is a defect in T cell or AM activation in patients with DM against TB infection. Objective: To study the difference in activation status of AM and T cells between patients with TB+DM and TB alone. Method: The heterogeneity of AM from 14 patients with TB+DM, 9 with TB alone, 10 normal subjects and 8 DM alone patients, was studied using Percoll density fractionation. The intracellular H2O2 production of AM before and after stimulation with phorbol myristate acetate (PMA) or F-Met-Leu-Phen (FMLP) was assayed by loading cells with 2',7'-dichlorofluorescin (DCFH) and analyzed by flow cytometry. Lymphocytes subsets (CD3, CD4, CD8) and their activation status (CD25) in bronchoalveolar lavage were also measured. Results: The proportion of the least dense AM (< 1.030 g/ml) and the magnitude of DCFH oxidation of AM was higher in TB patients than in normal subjects, regardless of DM. Patients with TB+DM had a significantly lower proportion of the least density AM fraction than TB alone patients, regardless of disease extent. Among TB patients, the proportion of the least dense AM was inversely correlated with the bacterial load on sputum and the disease extent on chest radiograph. Stimulation of AM with PMA or FMLP induced an increase in the hypodense AM subpopulations and enhanced intracellular H2O2 generation in patients with TB+DM and to a similar extent in normal subjects, but not in patients with TB alone. There was no significant difference in CD3 numbers, CD4/CD8 ratio, and CD25+ cells between patients with TB alone and TB+DM. The activation status of AM or T lymphocytes from DM alone patients was not significantly different from those from normal subjects. Conclusion: Hypodense subpopulations of AM increase in active TB patients and are related to the disease severity as well as activation status of AM. AM in TB patients complicated with DM was less activated, and may be contributory to the susceptibility to mycobacterial infection.

UR - http://www.scopus.com/inward/record.url?scp=0032848609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032848609&partnerID=8YFLogxK

U2 - 10.1054/tuld.1998.0167

DO - 10.1054/tuld.1998.0167

M3 - Article

C2 - 10692992

AN - SCOPUS:0032848609

VL - 79

SP - 235

EP - 242

JO - Tuberculosis

JF - Tuberculosis

SN - 1472-9792

IS - 4

ER -