Hyperthermia-induced controlled local anesthesia administration using gelatin-coated iron–gold alloy nanoparticles

Chien Kun Ting, Udesh Dhawan, Ching Li Tseng, Cihun Siyong Alex Gong, Wai Ching Liu, Huai De Tsai, Ren Jei Chung

Research output: Contribution to journalArticlepeer-review

Abstract

The lack of optimal methods employing nanoparticles to administer local anesthesia often results in posing severe risks such as non-biocompatibility, in vivo cytotoxicity, and drug overdose to patients. Here, we employed magnetic field-induced hyperthermia to achieve localized anesthesia. We synthesized iron–gold alloy nanoparticles (FeAu Nps), conjugated an anesthetic drug, Lidocaine, and coated the product with gelatin to increase the biocompatibility, resulting in a FeAu@Gelatin–Lidocaine nano-complex formation. The biocompatibility of this drug–nanoparticle conjugate was evaluated in vitro, and its ability to trigger local anesthesia was also evaluated in vivo. Upon exposure to high-frequency induction waves (HFIW), 7.2 ± 2.8 nm sized superparamagnetic nanoparticles generated heat, which dissociated the gelatin coating, thereby triggering Lidocaine release. MTT assay revealed that 82% of cells were viable at 5 mg/mL concentration of Lidocaine, indicating that no significant cytotoxicity was induced. In vivo experiments revealed that unless stimulated with HFIW, Lidocaine was not released from the FeAu@Gelatin–Lidocaine complex. In a proof-of-concept experiment, an intramuscular injection of FeAu@Gelatin–Lidocaine complex was administered to the rat posterior leg, which upon HFIW stimulation triggered an anesthetic effect to the injected muscle. Based on our findings, the FeAu@Gelatin–Lidocaine complex can deliver hyperthermia-induced controlled anesthetic drug release and serve as an ideal candidate for site-specific anesthesia administration.

Original languageEnglish
Article number1097
Pages (from-to)1-14
Number of pages14
JournalPharmaceutics
Volume12
Issue number11
DOIs
Publication statusPublished - Nov 2020

Keywords

  • Anesthesia
  • Hyperthermia
  • Iron–gold nanoparticles
  • Lidocaine
  • Nanoparticles

ASJC Scopus subject areas

  • Pharmaceutical Science

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