Hyperforin suppresses tumor growth and NF-ĸB-mediated anti-apoptotic and invasive potential of non-small cell lung cancer

Wei Ting Chen, Ying Kai Chen, Song Shei Lin, Fei Ting Hsu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background/Aim: Previous studies have indicated that hyperforin inhibits tumor growth of hepatocellular carcinoma. However, the anticancer effects of hyperforin in non-small cell lung cancer (NSCLC) are ambiguous. The aim of the present study was to investigate the anticancer effect of hyperforin in NSCLC. NSCLC CL1-5-F4 cells were treated with different concentrations of hyperforin or NF-ĸB inhibitor (QNZ) for different time periods. Materials and Methods: Change of cell viability, NF-ĸB activation, apoptotic signaling pathways, expression of anti-apoptotic proteins, and cell invasion were detected using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, NF-ĸB reporter gene assay, flow cytometry, western blotting, and cell invasion assay. Results: The results demonstrated that hyperforin significantly promotes extrinsic and intrinsic apoptotic pathways, and inhibits cell viability and NF-ĸB activation. In addition, results also indicated that blockage of NF-ĸB activation reduces the levels of anti-apoptotic proteins and cell invasion in CL1-5-F4 cells. Conclusion: These results suggested hyperforin induces apoptosis and inhibits NF-ĸB-modulated anti-apoptotic and invasive potential in NSCLC.

Original languageEnglish
Pages (from-to)2161-2167
Number of pages7
JournalAnticancer Research
Volume38
Issue number4
DOIs
Publication statusPublished - Apr 1 2018

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Non-Small Cell Lung Carcinoma
Growth
Neoplasms
Apoptosis Regulatory Proteins
Cell Survival
Gene Flow
Reporter Genes
hyperforin
Hepatocellular Carcinoma
Flow Cytometry
Western Blotting
Apoptosis

Keywords

  • Apoptosis
  • Hyperforin
  • Invasion.
  • NF-ĸB
  • Non-small cell lung cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Hyperforin suppresses tumor growth and NF-ĸB-mediated anti-apoptotic and invasive potential of non-small cell lung cancer. / Chen, Wei Ting; Chen, Ying Kai; Lin, Song Shei; Hsu, Fei Ting.

In: Anticancer Research, Vol. 38, No. 4, 01.04.2018, p. 2161-2167.

Research output: Contribution to journalArticle

Chen, Wei Ting ; Chen, Ying Kai ; Lin, Song Shei ; Hsu, Fei Ting. / Hyperforin suppresses tumor growth and NF-ĸB-mediated anti-apoptotic and invasive potential of non-small cell lung cancer. In: Anticancer Research. 2018 ; Vol. 38, No. 4. pp. 2161-2167.
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abstract = "Background/Aim: Previous studies have indicated that hyperforin inhibits tumor growth of hepatocellular carcinoma. However, the anticancer effects of hyperforin in non-small cell lung cancer (NSCLC) are ambiguous. The aim of the present study was to investigate the anticancer effect of hyperforin in NSCLC. NSCLC CL1-5-F4 cells were treated with different concentrations of hyperforin or NF-ĸB inhibitor (QNZ) for different time periods. Materials and Methods: Change of cell viability, NF-ĸB activation, apoptotic signaling pathways, expression of anti-apoptotic proteins, and cell invasion were detected using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, NF-ĸB reporter gene assay, flow cytometry, western blotting, and cell invasion assay. Results: The results demonstrated that hyperforin significantly promotes extrinsic and intrinsic apoptotic pathways, and inhibits cell viability and NF-ĸB activation. In addition, results also indicated that blockage of NF-ĸB activation reduces the levels of anti-apoptotic proteins and cell invasion in CL1-5-F4 cells. Conclusion: These results suggested hyperforin induces apoptosis and inhibits NF-ĸB-modulated anti-apoptotic and invasive potential in NSCLC.",
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