Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells

I. Tsang Chiang, Wei Ting Chen, Chih Wei Tseng, Yen Chung Chen, Yu Cheng Kuo, Bi Jhih Chen, Mao Chi Weng, Hwai Jeng Lin, Wei Shu Wang

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The aim of the present study was to investigate the antitumor effect and mechanism of action of hyperforin in hepatocellular carcinoma (HCC) SK-Hep1 cells in vitro. Cells were treated with different concentrations of hyperforin for different periods of time. Effects of hyperforin on cell viability, apoptosis signaling, and expression of anti-apoptotic and proliferative proteins [cellular FLICE-like inhibitory protein (c-FLIP), X-linked inhibitor of apoptosis protein (XIAP), myeloid cell leukemia 1(MCL1), and cyclin-D1] were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and western blotting. Hyperforin significantly inhibited cell viability and expression of anti-apoptotic and proliferative proteins. We also found that hyperforin significantly induced accumulation of cells in sub-G1 phase, loss of mitochondrial membrane potential, and increased levels of active caspase-3, and caspase-8. Taken together, our findings indicate that hyperforin triggers inhibition of tumor cell growth by inducing intrinsic and extrinsic apoptotic pathways in HCC SK-Hep1 cells.

Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalAnticancer Research
Volume37
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • Apoptosis
  • Hepatocellular carcinoma
  • Hyperforin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells'. Together they form a unique fingerprint.

Cite this