Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells

I. Tsang Chiang, Wei Ting Chen, Chih Wei Tseng, Yen Chung Chen, Yu Cheng Kuo, Bi Jhih Chen, Mao Chi Weng, Hwai Jeng Lin, Wei Shu Wang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The aim of the present study was to investigate the antitumor effect and mechanism of action of hyperforin in hepatocellular carcinoma (HCC) SK-Hep1 cells in vitro. Cells were treated with different concentrations of hyperforin for different periods of time. Effects of hyperforin on cell viability, apoptosis signaling, and expression of anti-apoptotic and proliferative proteins [cellular FLICE-like inhibitory protein (c-FLIP), X-linked inhibitor of apoptosis protein (XIAP), myeloid cell leukemia 1(MCL1), and cyclin-D1] were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and western blotting. Hyperforin significantly inhibited cell viability and expression of anti-apoptotic and proliferative proteins. We also found that hyperforin significantly induced accumulation of cells in sub-G1 phase, loss of mitochondrial membrane potential, and increased levels of active caspase-3, and caspase-8. Taken together, our findings indicate that hyperforin triggers inhibition of tumor cell growth by inducing intrinsic and extrinsic apoptotic pathways in HCC SK-Hep1 cells.

Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalAnticancer Research
Volume37
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Hepatocellular Carcinoma
Growth
Apoptosis Regulatory Proteins
Cell Survival
CASP8 and FADD-Like Apoptosis Regulating Protein
X-Linked Inhibitor of Apoptosis Protein
Myeloid Leukemia
Caspase 8
Mitochondrial Membrane Potential
Cyclin D1
G1 Phase
Myeloid Cells
Caspase 3
hyperforin
Flow Cytometry
Western Blotting
Apoptosis
Neoplasms

Keywords

  • Apoptosis
  • Hepatocellular carcinoma
  • Hyperforin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Chiang, I. T., Chen, W. T., Tseng, C. W., Chen, Y. C., Kuo, Y. C., Chen, B. J., ... Wang, W. S. (2017). Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells. Anticancer Research, 37(1), 161-167. https://doi.org/10.21873/anticanres.11301

Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells. / Chiang, I. Tsang; Chen, Wei Ting; Tseng, Chih Wei; Chen, Yen Chung; Kuo, Yu Cheng; Chen, Bi Jhih; Weng, Mao Chi; Lin, Hwai Jeng; Wang, Wei Shu.

In: Anticancer Research, Vol. 37, No. 1, 01.01.2017, p. 161-167.

Research output: Contribution to journalArticle

Chiang, I. Tsang ; Chen, Wei Ting ; Tseng, Chih Wei ; Chen, Yen Chung ; Kuo, Yu Cheng ; Chen, Bi Jhih ; Weng, Mao Chi ; Lin, Hwai Jeng ; Wang, Wei Shu. / Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells. In: Anticancer Research. 2017 ; Vol. 37, No. 1. pp. 161-167.
@article{67d461cd9d224bb2bb9c9de703579856,
title = "Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells",
abstract = "The aim of the present study was to investigate the antitumor effect and mechanism of action of hyperforin in hepatocellular carcinoma (HCC) SK-Hep1 cells in vitro. Cells were treated with different concentrations of hyperforin for different periods of time. Effects of hyperforin on cell viability, apoptosis signaling, and expression of anti-apoptotic and proliferative proteins [cellular FLICE-like inhibitory protein (c-FLIP), X-linked inhibitor of apoptosis protein (XIAP), myeloid cell leukemia 1(MCL1), and cyclin-D1] were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and western blotting. Hyperforin significantly inhibited cell viability and expression of anti-apoptotic and proliferative proteins. We also found that hyperforin significantly induced accumulation of cells in sub-G1 phase, loss of mitochondrial membrane potential, and increased levels of active caspase-3, and caspase-8. Taken together, our findings indicate that hyperforin triggers inhibition of tumor cell growth by inducing intrinsic and extrinsic apoptotic pathways in HCC SK-Hep1 cells.",
keywords = "Apoptosis, Hepatocellular carcinoma, Hyperforin",
author = "Chiang, {I. Tsang} and Chen, {Wei Ting} and Tseng, {Chih Wei} and Chen, {Yen Chung} and Kuo, {Yu Cheng} and Chen, {Bi Jhih} and Weng, {Mao Chi} and Lin, {Hwai Jeng} and Wang, {Wei Shu}",
year = "2017",
month = "1",
day = "1",
doi = "10.21873/anticanres.11301",
language = "English",
volume = "37",
pages = "161--167",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "1",

}

TY - JOUR

T1 - Hyperforin inhibits cell growth by inducing intrinsic and extrinsic apoptotic pathways in Hepatocellular carcinoma cells

AU - Chiang, I. Tsang

AU - Chen, Wei Ting

AU - Tseng, Chih Wei

AU - Chen, Yen Chung

AU - Kuo, Yu Cheng

AU - Chen, Bi Jhih

AU - Weng, Mao Chi

AU - Lin, Hwai Jeng

AU - Wang, Wei Shu

PY - 2017/1/1

Y1 - 2017/1/1

N2 - The aim of the present study was to investigate the antitumor effect and mechanism of action of hyperforin in hepatocellular carcinoma (HCC) SK-Hep1 cells in vitro. Cells were treated with different concentrations of hyperforin for different periods of time. Effects of hyperforin on cell viability, apoptosis signaling, and expression of anti-apoptotic and proliferative proteins [cellular FLICE-like inhibitory protein (c-FLIP), X-linked inhibitor of apoptosis protein (XIAP), myeloid cell leukemia 1(MCL1), and cyclin-D1] were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and western blotting. Hyperforin significantly inhibited cell viability and expression of anti-apoptotic and proliferative proteins. We also found that hyperforin significantly induced accumulation of cells in sub-G1 phase, loss of mitochondrial membrane potential, and increased levels of active caspase-3, and caspase-8. Taken together, our findings indicate that hyperforin triggers inhibition of tumor cell growth by inducing intrinsic and extrinsic apoptotic pathways in HCC SK-Hep1 cells.

AB - The aim of the present study was to investigate the antitumor effect and mechanism of action of hyperforin in hepatocellular carcinoma (HCC) SK-Hep1 cells in vitro. Cells were treated with different concentrations of hyperforin for different periods of time. Effects of hyperforin on cell viability, apoptosis signaling, and expression of anti-apoptotic and proliferative proteins [cellular FLICE-like inhibitory protein (c-FLIP), X-linked inhibitor of apoptosis protein (XIAP), myeloid cell leukemia 1(MCL1), and cyclin-D1] were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and western blotting. Hyperforin significantly inhibited cell viability and expression of anti-apoptotic and proliferative proteins. We also found that hyperforin significantly induced accumulation of cells in sub-G1 phase, loss of mitochondrial membrane potential, and increased levels of active caspase-3, and caspase-8. Taken together, our findings indicate that hyperforin triggers inhibition of tumor cell growth by inducing intrinsic and extrinsic apoptotic pathways in HCC SK-Hep1 cells.

KW - Apoptosis

KW - Hepatocellular carcinoma

KW - Hyperforin

UR - http://www.scopus.com/inward/record.url?scp=85007524266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007524266&partnerID=8YFLogxK

U2 - 10.21873/anticanres.11301

DO - 10.21873/anticanres.11301

M3 - Article

C2 - 28011486

AN - SCOPUS:85007524266

VL - 37

SP - 161

EP - 167

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 1

ER -