Hyperbaric oxygen upregulates cochlear constitutive nitric oxide synthase

Chia Der Lin, I. Hua Wei, Chih Ho Lai, Te Chun Hsia, Ming Ching Kao, Ming Hsui Tsai, Ching Hsiang Wu, Mang Hung Tsai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Hyperbaric oxygen therapy (HBOT) is a known adjuvant for treating ischemia-related inner ear diseases. Controversies still exist in the role of HBOT in cochlear diseases. Few studies to date have investigated the cellular changes that occur in inner ears after HBOT. Nitric oxide, which is synthesized by nitric oxide synthase (NOS), is an important signaling molecule in cochlear physiology and pathology. Here we investigated the effects of hyperbaric oxygen on eardrum morphology, cochlear function and expression of NOS isoforms in cochlear substructures after repetitive HBOT in guinea pigs.Results: Minor changes in the eardrum were observed after repetitive HBOT, which did not result in a significant hearing threshold shift by tone burst auditory brainstem responses. A differential effect of HBOT on the expression of NOS isoforms was identified. Upregulation of constitutive NOS (nNOS and eNOS) was found in the substructures of the cochlea after HBOT, but inducible NOS was not found in normal or HBOT animals, as shown by immunohistochemistry. There was no obvious DNA fragmentation present in this HBOT animal model.Conclusions: The present evidence indicates that the customary HBOT protocol may increase constitutive NOS expression but such upregulation did not cause cell death in the treated cochlea. The cochlear morphology and auditory function are consequently not changed through the protocol.

Original languageEnglish
Article number21
JournalBMC Neuroscience
Volume12
DOIs
Publication statusPublished - Feb 22 2011
Externally publishedYes

Fingerprint

Hyperbaric Oxygenation
Cochlea
Nitric Oxide Synthase
Up-Regulation
Oxygen
Tympanic Membrane
Cochlear Diseases
Protein Isoforms
Labyrinth Diseases
Brain Stem Auditory Evoked Potentials
Nitric Oxide Synthase Type II
DNA Fragmentation
Inner Ear
Hearing
Cause of Death
Nitric Oxide
Guinea Pigs
Cell Death
Ischemia
Animal Models

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neuroscience(all)

Cite this

Lin, C. D., Wei, I. H., Lai, C. H., Hsia, T. C., Kao, M. C., Tsai, M. H., ... Tsai, M. H. (2011). Hyperbaric oxygen upregulates cochlear constitutive nitric oxide synthase. BMC Neuroscience, 12, [21]. https://doi.org/10.1186/1471-2202-12-21

Hyperbaric oxygen upregulates cochlear constitutive nitric oxide synthase. / Lin, Chia Der; Wei, I. Hua; Lai, Chih Ho; Hsia, Te Chun; Kao, Ming Ching; Tsai, Ming Hsui; Wu, Ching Hsiang; Tsai, Mang Hung.

In: BMC Neuroscience, Vol. 12, 21, 22.02.2011.

Research output: Contribution to journalArticle

Lin, CD, Wei, IH, Lai, CH, Hsia, TC, Kao, MC, Tsai, MH, Wu, CH & Tsai, MH 2011, 'Hyperbaric oxygen upregulates cochlear constitutive nitric oxide synthase', BMC Neuroscience, vol. 12, 21. https://doi.org/10.1186/1471-2202-12-21
Lin, Chia Der ; Wei, I. Hua ; Lai, Chih Ho ; Hsia, Te Chun ; Kao, Ming Ching ; Tsai, Ming Hsui ; Wu, Ching Hsiang ; Tsai, Mang Hung. / Hyperbaric oxygen upregulates cochlear constitutive nitric oxide synthase. In: BMC Neuroscience. 2011 ; Vol. 12.
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