Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells

Shankung Lin, Kou-Gi Shyu, Chun Chung Lee, Bao Wei Wang, Chih Chuan Chang, Ya Chen Liu, Fan Yen Huang, Hang Chang

Research output: Contribution to journalArticle

35 Citations (Scopus)


One of the biological effects of hyperbaric oxygen (HBO) therapy in enhancing ischemia-related wound healing is the induction of angiogenesis. To elucidate the mechanism(s) underlying the HBO-induced angiogenesis, we studied the expression of several angiogenesis-related genes in human umbilical vein endothelial cells exposed to HBO. Western blot analyses showed that HBO enhanced the expression of angiopoietin-2 (Ang2) with no effect on the expression of Tie2, angiopoietin-1, and VEGF. The induction of Ang2 was further confirmed by immunohistochemistry, quantitative PCR, and Northern blot analyses. Inhibition of endothelial nitric oxide synthase blocked the HBO-induced Ang2 expression, but failed to block hypoxia-induced Ang2 expression. These data indicated that HBO-induced Ang2 expression may be through transcriptional stimulation, and requires the nitric oxide signaling pathway, which may play an important role in HBO-induced angiogenesis.

Original languageEnglish
Pages (from-to)710-715
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 2002



  • Angiopoietin
  • Human umbilical vein endothelial cells
  • Hyperbaric oxygen
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Lin, S., Shyu, K-G., Lee, C. C., Wang, B. W., Chang, C. C., Liu, Y. C., Huang, F. Y., & Chang, H. (2002). Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells. Biochemical and Biophysical Research Communications, 296(3), 710-715. https://doi.org/10.1016/S0006-291X(02)00924-5