Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells

Shankung Lin, Kou-Gi Shyu, Chun Chung Lee, Bao Wei Wang, Chih Chuan Chang, Ya Chen Liu, Fan Yen Huang, Hang Chang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

One of the biological effects of hyperbaric oxygen (HBO) therapy in enhancing ischemia-related wound healing is the induction of angiogenesis. To elucidate the mechanism(s) underlying the HBO-induced angiogenesis, we studied the expression of several angiogenesis-related genes in human umbilical vein endothelial cells exposed to HBO. Western blot analyses showed that HBO enhanced the expression of angiopoietin-2 (Ang2) with no effect on the expression of Tie2, angiopoietin-1, and VEGF. The induction of Ang2 was further confirmed by immunohistochemistry, quantitative PCR, and Northern blot analyses. Inhibition of endothelial nitric oxide synthase blocked the HBO-induced Ang2 expression, but failed to block hypoxia-induced Ang2 expression. These data indicated that HBO-induced Ang2 expression may be through transcriptional stimulation, and requires the nitric oxide signaling pathway, which may play an important role in HBO-induced angiogenesis.

Original languageEnglish
Pages (from-to)710-715
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume296
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Angiopoietin-2
Endothelial cells
Human Umbilical Vein Endothelial Cells
Oxygen
Angiopoietin-1
Hyperbaric Oxygenation
Nitric Oxide Synthase Type III
Northern Blotting
Wound Healing
Vascular Endothelial Growth Factor A
Nitric Oxide
Ischemia
Western Blotting
Immunohistochemistry
Genes
Polymerase Chain Reaction

Keywords

  • Angiopoietin
  • Human umbilical vein endothelial cells
  • Hyperbaric oxygen
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells. / Lin, Shankung; Shyu, Kou-Gi; Lee, Chun Chung; Wang, Bao Wei; Chang, Chih Chuan; Liu, Ya Chen; Huang, Fan Yen; Chang, Hang.

In: Biochemical and Biophysical Research Communications, Vol. 296, No. 3, 2002, p. 710-715.

Research output: Contribution to journalArticle

Lin, Shankung ; Shyu, Kou-Gi ; Lee, Chun Chung ; Wang, Bao Wei ; Chang, Chih Chuan ; Liu, Ya Chen ; Huang, Fan Yen ; Chang, Hang. / Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 296, No. 3. pp. 710-715.
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