Hyperbaric oxygen induces placental growth factor expression in bone marrow-derived mesenchymal stem cells

Kou-Gi Shyu, Huei Fong Hung, Bao Wei Wang, Hang Chang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The bone marrow is home to mesenchymal stem cells (MSCs) that are able to differentiate into many different cell types. The effect of hyperbaric oxygen (HBO) on MSCs is poorly understood. Placental growth factor (PlGF) is an attractive therapeutic agent for stimulating revascularization of ischemic tissue. HBO has been shown to improve diabetic wound healing by increase circulating stem cells. We hypothesized that HBO induces PlGF expression in bone marrow-derived MSCs. The MSCs were obtained from adult human bone marrow and expanded in vitro. The purity and characteristics of MSCs were identified by flow cytometry and immunophenotyping. HBO at 2.5 ATA (atmosphere absolute) significantly increased PlGF protein and mRNA expression. The induction of PlGF protein by HBO was significantly blocked by the addition of N-acetylcysteine, while wortmannin, PD98059, SP600125 and SB203580 had no effect on PlGF protein expression. However, the specific inhibitor of nitric oxide synthase, L-NAME did not alter the PlGF protein expression induced by HBO. HBO significantly increased the reactive oxygen species production and pretreatment with N-acetylcysteine significantly blocked the induction of reactive oxygen species by HBO. HBO significantly increased the migration and tube formation of MSCs and pretreatment with N-acetylcysteine and PlGF siRNA significantly blocked the induction of migration and tube formation by HBO. In conclusion, HBO induced the expression of PlGF in human bone marrow-derived MSCs at least through the oxidative stress-related pathways, which may play an important role in HBO-induced vasculogenesis.

Original languageEnglish
Pages (from-to)65-73
Number of pages9
JournalLife Sciences
Volume83
Issue number1-2
DOIs
Publication statusPublished - Jul 4 2008

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Intercellular Signaling Peptides and Proteins
Bone
Bone Marrow
Oxygen
Acetylcysteine
Reactive Oxygen Species
Proteins
Immunophenotyping
Oxidative stress
Flow cytometry
NG-Nitroarginine Methyl Ester
Atmosphere
Nitric Oxide Synthase
Wound Healing
Small Interfering RNA
Flow Cytometry
Oxidative Stress
Stem Cells

Keywords

  • Hyperbaric oxygen
  • Oxidative stress
  • Reactive oxygen species
  • Stem cell

ASJC Scopus subject areas

  • Pharmacology

Cite this

Hyperbaric oxygen induces placental growth factor expression in bone marrow-derived mesenchymal stem cells. / Shyu, Kou-Gi; Hung, Huei Fong; Wang, Bao Wei; Chang, Hang.

In: Life Sciences, Vol. 83, No. 1-2, 04.07.2008, p. 65-73.

Research output: Contribution to journalArticle

Shyu, Kou-Gi ; Hung, Huei Fong ; Wang, Bao Wei ; Chang, Hang. / Hyperbaric oxygen induces placental growth factor expression in bone marrow-derived mesenchymal stem cells. In: Life Sciences. 2008 ; Vol. 83, No. 1-2. pp. 65-73.
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