Hylan G-F 20 attenuates posttraumatic osteoarthritis progression: Association with upregulated expression of the circardian gene NPAS2

Chih Chung Liu, Li Jen Su, Wei Yuan Tsai, Hsiao Lun Sun, Hoong Chien Lee, Chih Shung Wong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aims The study was to examine the effect of Hylan G-F 20 on the progression of posttraumatic osteoarthritis (PTOA) and the expression of the circadian genes neuronal PAS domain protein 2 (NPAS2) and period 2 (Per2). Main methods We used the anterior cruciate ligament transaction and medial menisectomy (ACLT + MMx) model in Wistar rats. The rats were divided into three groups, the sham-operated group, the Hylan G-F 20-treated group, and the saline-treated group. Rats which underwent ACLT + MMx surgery were injected intraarticularly with, respectively, Hylan G-F 20 or saline once a week for 3 consecutive weeks, starting 7 days after surgery. The gross morphology and histopathology of the experimental knee joints were evaluated at the end of week 6. Expression of the NPAS2 and Per2 genes was measured by real-time PCR. Key findings Hylan G-F 20 suppressed the articular cartilage destruction and synovitis compared to the saline-treated group. Compared to the sham-operated group, the Hylan G-F 20-treated group showed significantly upregulated expression of NPAS2 in cartilage (2.53 ± 0.08-fold higher; p < 0.05) and a non-significant increase in Per2 expression (2.35 ± 1.26-fold higher p = 0.28), while the saline-treated group showed significant downregulation of NPAS2 expression and a non-significant decrease in Per2 expression. Significance Our data suggested that early intraarticular injection of Hylan G-F 20 attenuates the progression of PTOA and significantly upregulates NPAS2 expression. These findings provide a new direction for studying associations between the use of a pharmacological agent, the degenerative process, and circadian gene expression.

Original languageEnglish
Article number14494
Pages (from-to)20-24
Number of pages5
JournalLife Sciences
Volume141
DOIs
Publication statusPublished - Nov 15 2015

Fingerprint

Osteoarthritis
Genes
Gene Expression
Period Circadian Proteins
Rats
Proteins
Cartilage
Surgery
Intra-Articular Injections
Synovitis
Anterior Cruciate Ligament
Ligaments
Articular Cartilage
Knee Joint
Ambulatory Surgical Procedures
Gene expression
hylan
Protein Domains
Wistar Rats
Real-Time Polymerase Chain Reaction

Keywords

  • Arthritis
  • Circadian rhythm
  • Hyaluronic acid
  • Hylan
  • NPAS2

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Hylan G-F 20 attenuates posttraumatic osteoarthritis progression : Association with upregulated expression of the circardian gene NPAS2. / Liu, Chih Chung; Su, Li Jen; Tsai, Wei Yuan; Sun, Hsiao Lun; Lee, Hoong Chien; Wong, Chih Shung.

In: Life Sciences, Vol. 141, 14494, 15.11.2015, p. 20-24.

Research output: Contribution to journalArticle

Liu, Chih Chung ; Su, Li Jen ; Tsai, Wei Yuan ; Sun, Hsiao Lun ; Lee, Hoong Chien ; Wong, Chih Shung. / Hylan G-F 20 attenuates posttraumatic osteoarthritis progression : Association with upregulated expression of the circardian gene NPAS2. In: Life Sciences. 2015 ; Vol. 141. pp. 20-24.
@article{718a88d3ffda40ee985b5c49c5c6d290,
title = "Hylan G-F 20 attenuates posttraumatic osteoarthritis progression: Association with upregulated expression of the circardian gene NPAS2",
abstract = "Aims The study was to examine the effect of Hylan G-F 20 on the progression of posttraumatic osteoarthritis (PTOA) and the expression of the circadian genes neuronal PAS domain protein 2 (NPAS2) and period 2 (Per2). Main methods We used the anterior cruciate ligament transaction and medial menisectomy (ACLT + MMx) model in Wistar rats. The rats were divided into three groups, the sham-operated group, the Hylan G-F 20-treated group, and the saline-treated group. Rats which underwent ACLT + MMx surgery were injected intraarticularly with, respectively, Hylan G-F 20 or saline once a week for 3 consecutive weeks, starting 7 days after surgery. The gross morphology and histopathology of the experimental knee joints were evaluated at the end of week 6. Expression of the NPAS2 and Per2 genes was measured by real-time PCR. Key findings Hylan G-F 20 suppressed the articular cartilage destruction and synovitis compared to the saline-treated group. Compared to the sham-operated group, the Hylan G-F 20-treated group showed significantly upregulated expression of NPAS2 in cartilage (2.53 ± 0.08-fold higher; p < 0.05) and a non-significant increase in Per2 expression (2.35 ± 1.26-fold higher p = 0.28), while the saline-treated group showed significant downregulation of NPAS2 expression and a non-significant decrease in Per2 expression. Significance Our data suggested that early intraarticular injection of Hylan G-F 20 attenuates the progression of PTOA and significantly upregulates NPAS2 expression. These findings provide a new direction for studying associations between the use of a pharmacological agent, the degenerative process, and circadian gene expression.",
keywords = "Arthritis, Circadian rhythm, Hyaluronic acid, Hylan, NPAS2",
author = "Liu, {Chih Chung} and Su, {Li Jen} and Tsai, {Wei Yuan} and Sun, {Hsiao Lun} and Lee, {Hoong Chien} and Wong, {Chih Shung}",
year = "2015",
month = "11",
day = "15",
doi = "10.1016/j.lfs.2015.09.007",
language = "English",
volume = "141",
pages = "20--24",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Hylan G-F 20 attenuates posttraumatic osteoarthritis progression

T2 - Association with upregulated expression of the circardian gene NPAS2

AU - Liu, Chih Chung

AU - Su, Li Jen

AU - Tsai, Wei Yuan

AU - Sun, Hsiao Lun

AU - Lee, Hoong Chien

AU - Wong, Chih Shung

PY - 2015/11/15

Y1 - 2015/11/15

N2 - Aims The study was to examine the effect of Hylan G-F 20 on the progression of posttraumatic osteoarthritis (PTOA) and the expression of the circadian genes neuronal PAS domain protein 2 (NPAS2) and period 2 (Per2). Main methods We used the anterior cruciate ligament transaction and medial menisectomy (ACLT + MMx) model in Wistar rats. The rats were divided into three groups, the sham-operated group, the Hylan G-F 20-treated group, and the saline-treated group. Rats which underwent ACLT + MMx surgery were injected intraarticularly with, respectively, Hylan G-F 20 or saline once a week for 3 consecutive weeks, starting 7 days after surgery. The gross morphology and histopathology of the experimental knee joints were evaluated at the end of week 6. Expression of the NPAS2 and Per2 genes was measured by real-time PCR. Key findings Hylan G-F 20 suppressed the articular cartilage destruction and synovitis compared to the saline-treated group. Compared to the sham-operated group, the Hylan G-F 20-treated group showed significantly upregulated expression of NPAS2 in cartilage (2.53 ± 0.08-fold higher; p < 0.05) and a non-significant increase in Per2 expression (2.35 ± 1.26-fold higher p = 0.28), while the saline-treated group showed significant downregulation of NPAS2 expression and a non-significant decrease in Per2 expression. Significance Our data suggested that early intraarticular injection of Hylan G-F 20 attenuates the progression of PTOA and significantly upregulates NPAS2 expression. These findings provide a new direction for studying associations between the use of a pharmacological agent, the degenerative process, and circadian gene expression.

AB - Aims The study was to examine the effect of Hylan G-F 20 on the progression of posttraumatic osteoarthritis (PTOA) and the expression of the circadian genes neuronal PAS domain protein 2 (NPAS2) and period 2 (Per2). Main methods We used the anterior cruciate ligament transaction and medial menisectomy (ACLT + MMx) model in Wistar rats. The rats were divided into three groups, the sham-operated group, the Hylan G-F 20-treated group, and the saline-treated group. Rats which underwent ACLT + MMx surgery were injected intraarticularly with, respectively, Hylan G-F 20 or saline once a week for 3 consecutive weeks, starting 7 days after surgery. The gross morphology and histopathology of the experimental knee joints were evaluated at the end of week 6. Expression of the NPAS2 and Per2 genes was measured by real-time PCR. Key findings Hylan G-F 20 suppressed the articular cartilage destruction and synovitis compared to the saline-treated group. Compared to the sham-operated group, the Hylan G-F 20-treated group showed significantly upregulated expression of NPAS2 in cartilage (2.53 ± 0.08-fold higher; p < 0.05) and a non-significant increase in Per2 expression (2.35 ± 1.26-fold higher p = 0.28), while the saline-treated group showed significant downregulation of NPAS2 expression and a non-significant decrease in Per2 expression. Significance Our data suggested that early intraarticular injection of Hylan G-F 20 attenuates the progression of PTOA and significantly upregulates NPAS2 expression. These findings provide a new direction for studying associations between the use of a pharmacological agent, the degenerative process, and circadian gene expression.

KW - Arthritis

KW - Circadian rhythm

KW - Hyaluronic acid

KW - Hylan

KW - NPAS2

UR - http://www.scopus.com/inward/record.url?scp=84943175987&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943175987&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2015.09.007

DO - 10.1016/j.lfs.2015.09.007

M3 - Article

C2 - 26388558

AN - SCOPUS:84943175987

VL - 141

SP - 20

EP - 24

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

M1 - 14494

ER -