Hydrogen Sulfide modulates the S-Nitrosoproteome and the mitochondrial morphology in endothelial cells

Tsan Wan Chiu, Ying Lun Chen, Chien Yi Wu, Pei Ling Yu, Ying Hua Shieh, Bin Huang

Research output: Contribution to journalArticle

Abstract

Background: Hydrogen sulfide (H2S) is one of the endogenous gaseous molecules promoting the production of nitric oxide (NO) which has cardioprotective functions. However, the role of the H2S-mediated protein Snitrosoproteome and its subsequent physiological response remains unclear. Methods: Endothelial cells EAhy 926 were treated with 50 μMof H2S for 2 hours. The NO bound S-nitrosoproteins were purified by a biotin-switch and then digested by trypsin. Resulting peptides from control and H2S treatment were separately labeled by isobaric tag for relative and absolute quantitation 114/115, quantified by liquid chromatography tandem-mass spectrometry and analyzed by ingenuity pathway analysis (IPA) software. The microP software was applied to analyze the morphological changes of mitochondria. Results: With the treatment of H2S, 416 S-nitrosylated proteins were identified. IPA analysis showed that these proteins were involved in five signaling pathways. The NO-bound cysteine residues and the S-nitrosylation levels (115/114) were shown for ten S-nitrosoproteins. Western blot further verified the S-nitrosylation of thioredoxindependant peroxide reductase, cytochrome c oxidase and cytochrome b-c1 complex that are involved in the mitochondrial signaling pathway. H2O2-induced mitochondrial swelling can be reduced by the pretreatment of H2S. Conclusions: The H2S-mediated endothelial S-nitrosoproteome has been confirmed. In the present study, we have proposed the cardioprotective role of H2S via maintaining mitochondrial homeostasis.

Original languageEnglish
Pages (from-to)604-611
Number of pages8
JournalActa Cardiologica Sinica
Volume32
Issue number5
DOIs
Publication statusPublished - Sep 1 2016

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Hydrogen Sulfide
Nitric Oxide
Endothelial Cells
Software
Far-Western Blotting
Cytochromes c1
Mitochondrial Swelling
Cytochromes b
Protein S
Peroxides
Electron Transport Complex IV
Biotin
Tandem Mass Spectrometry
Liquid Chromatography
Trypsin
Cysteine
Mitochondria
Oxidoreductases
Proteins
Homeostasis

Keywords

  • Endothelial cell
  • Hydrogen sulfide
  • MicroP
  • Mitochondria
  • Mitophagy
  • S-nitrosoproteome

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Hydrogen Sulfide modulates the S-Nitrosoproteome and the mitochondrial morphology in endothelial cells. / Chiu, Tsan Wan; Chen, Ying Lun; Wu, Chien Yi; Yu, Pei Ling; Shieh, Ying Hua; Huang, Bin.

In: Acta Cardiologica Sinica, Vol. 32, No. 5, 01.09.2016, p. 604-611.

Research output: Contribution to journalArticle

Chiu, Tsan Wan ; Chen, Ying Lun ; Wu, Chien Yi ; Yu, Pei Ling ; Shieh, Ying Hua ; Huang, Bin. / Hydrogen Sulfide modulates the S-Nitrosoproteome and the mitochondrial morphology in endothelial cells. In: Acta Cardiologica Sinica. 2016 ; Vol. 32, No. 5. pp. 604-611.
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