This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60μm) ratio higher than 60%, suitable for in-vitro fertilization (IVF).
|Title of host publication||2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016|
|Number of pages||4|
|Publication status||Published - Nov 28 2016|
Chen, Y. J., Wu, R. G., Chen, P. C., Lin, Y. N., Hsiu, H. W., Yen, C. C., Wang, C-W., Pan, L-C., & Tseng, F. G. (2016). Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 (pp. 130-133) https://doi.org/10.1109/NEMS.2016.7758215