Hyaluronan regulates PPARγ and inflammatory responses in IL-1β-stimulated human chondrosarcoma cells, a model for osteoarthritis

Chi Ching Chang, Ming-Shium Hsieh, Say Tsung Liao, Yi Hsuan Chen, Chao Wen Cheng, Pai Tsang Huang, Yung Feng Lin, Chien Ho Chen

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27 Citations (Scopus)

Abstract

The carbohydrate polymer, hyaluronan, is a major component of the extracellular matrix in animal tissues. Exogenous hyaluronan has been used to treat osteoarthritis (OA), a degenerative joint disease involving inflammatory changes. The underlying mechanisms of hyaluronan in OA are not fully understood. Pro-inflammatory interleukin (IL)-1β downregulates peroxisome proliferator-activated receptor gamma (PPARγ), and increases expression of matrix metalloproteinases (MMPs) which are responsible for the degeneration of articular cartilage. The effects of low- and high-molecular-weight hyaluronan (oligo-HA and HMW-HA) on the inflammatory genes were determined in human SW-1353 chondrosarcoma cells. HMW-HA antagonized the effects of IL-1β by increasing PPARγ and decreasing cyclooxygenase (COX)-2, MMP-1, and MMP-13 levels. It promoted Akt, but suppressed mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NFκB) signaling, indicating anti-inflammatory effects. In contrast, the cells had overall opposite responses to oligo-HA. In conclusion, HMW-HA and oligo-HA exerted differential inflammatory responses via PPARγ in IL-1β-treated chondrosarcoma cells.

Original languageEnglish
Pages (from-to)1168-1175
Number of pages8
JournalCarbohydrate Polymers
Volume90
Issue number2
DOIs
Publication statusPublished - Oct 1 2012

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Keywords

  • Cyclooxygenase-2
  • Hyaluronan
  • Matrix metalloproteinase
  • Osteoarthritis
  • Peroxisome proliferator-activated receptor

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

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