Hyaluronan regulates PPARγ and inflammatory responses in IL-1β-stimulated human chondrosarcoma cells, a model for osteoarthritis

Chi Ching Chang, Ming-Shium Hsieh, Say Tsung Liao, Yi Hsuan Chen, Chao Wen Cheng, Pai Tsang Huang, Yung Feng Lin, Chien Ho Chen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The carbohydrate polymer, hyaluronan, is a major component of the extracellular matrix in animal tissues. Exogenous hyaluronan has been used to treat osteoarthritis (OA), a degenerative joint disease involving inflammatory changes. The underlying mechanisms of hyaluronan in OA are not fully understood. Pro-inflammatory interleukin (IL)-1β downregulates peroxisome proliferator-activated receptor gamma (PPARγ), and increases expression of matrix metalloproteinases (MMPs) which are responsible for the degeneration of articular cartilage. The effects of low- and high-molecular-weight hyaluronan (oligo-HA and HMW-HA) on the inflammatory genes were determined in human SW-1353 chondrosarcoma cells. HMW-HA antagonized the effects of IL-1β by increasing PPARγ and decreasing cyclooxygenase (COX)-2, MMP-1, and MMP-13 levels. It promoted Akt, but suppressed mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NFκB) signaling, indicating anti-inflammatory effects. In contrast, the cells had overall opposite responses to oligo-HA. In conclusion, HMW-HA and oligo-HA exerted differential inflammatory responses via PPARγ in IL-1β-treated chondrosarcoma cells.

Original languageEnglish
Pages (from-to)1168-1175
Number of pages8
JournalCarbohydrate Polymers
Volume90
Issue number2
DOIs
Publication statusPublished - Oct 1 2012

Fingerprint

PPAR gamma
Hyaluronic Acid
Interleukin-1
Cartilage
Carbohydrates
Matrix Metalloproteinase 13
Matrix Metalloproteinase 1
NF-kappa B
Animals
Cyclooxygenase 2
Genes
Mitogen-Activated Protein Kinases
Matrix Metalloproteinases
Molecular weight
Tissue
Proteins
Polymers
Anti-Inflammatory Agents
Peroxisome Proliferator-Activated Receptors
Metalloproteases

Keywords

  • Cyclooxygenase-2
  • Hyaluronan
  • Matrix metalloproteinase
  • Osteoarthritis
  • Peroxisome proliferator-activated receptor

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Hyaluronan regulates PPARγ and inflammatory responses in IL-1β-stimulated human chondrosarcoma cells, a model for osteoarthritis. / Chang, Chi Ching; Hsieh, Ming-Shium; Liao, Say Tsung; Chen, Yi Hsuan; Cheng, Chao Wen; Huang, Pai Tsang; Lin, Yung Feng; Chen, Chien Ho.

In: Carbohydrate Polymers, Vol. 90, No. 2, 01.10.2012, p. 1168-1175.

Research output: Contribution to journalArticle

Chang, Chi Ching ; Hsieh, Ming-Shium ; Liao, Say Tsung ; Chen, Yi Hsuan ; Cheng, Chao Wen ; Huang, Pai Tsang ; Lin, Yung Feng ; Chen, Chien Ho. / Hyaluronan regulates PPARγ and inflammatory responses in IL-1β-stimulated human chondrosarcoma cells, a model for osteoarthritis. In: Carbohydrate Polymers. 2012 ; Vol. 90, No. 2. pp. 1168-1175.
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