HUMN project: Detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures

Michael Fenech, W. P. Chang, M. Kirsch-Volders, N. Holland, S. Bonassi, E. Zeiger

Research output: Contribution to journalArticle

876 Citations (Scopus)

Abstract

Criteria for scoring micronuclei and nucleoplasmic bridges in binucleated cells in the cytokinesis-block micronucleus assay for isolated human lymphocyte cultures are described in detail. Morphological characteristics of mononucleated cells, binucleated cells, and multinucleated cells as well as necrotic and apoptotic cells and nuclear buds are also described. These criteria are illustrated by a series of schematic diagrams as well as a comprehensive set of colour photographs that are of practical assistance during the scoring of slides. These scoring criteria, diagrams and photographs have been used in a HUman MicronNucleus (HUMN) project inter-laboratory slide-scoring exercise to evaluate the extent of variability that can be attributable to individual scorers and individual laboratories when measuring the frequency of micronuclei and nucleoplasmic bridges in binucleated cells as well as the nuclear division index. The results of the latter study are described in an accompanying paper. It is expected that these scoring criteria will assist in the development of a procedure for calibrating scorers and laboratories so that results from different laboratories for the cytokinesis-block micronucleus assay may be more comparable in the future.

Original languageEnglish
Pages (from-to)65-75
Number of pages11
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume534
Issue number1-2
DOIs
Publication statusPublished - Jan 10 2003
Externally publishedYes

Fingerprint

Micronucleus Tests
Cytokinesis
Lymphocytes
Cell Nucleus Division
Color

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Genetics

Cite this

HUMN project : Detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. / Fenech, Michael; Chang, W. P.; Kirsch-Volders, M.; Holland, N.; Bonassi, S.; Zeiger, E.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 534, No. 1-2, 10.01.2003, p. 65-75.

Research output: Contribution to journalArticle

@article{474abcb1dc98438fa86ac7a6720c9d9f,
title = "HUMN project: Detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures",
abstract = "Criteria for scoring micronuclei and nucleoplasmic bridges in binucleated cells in the cytokinesis-block micronucleus assay for isolated human lymphocyte cultures are described in detail. Morphological characteristics of mononucleated cells, binucleated cells, and multinucleated cells as well as necrotic and apoptotic cells and nuclear buds are also described. These criteria are illustrated by a series of schematic diagrams as well as a comprehensive set of colour photographs that are of practical assistance during the scoring of slides. These scoring criteria, diagrams and photographs have been used in a HUman MicronNucleus (HUMN) project inter-laboratory slide-scoring exercise to evaluate the extent of variability that can be attributable to individual scorers and individual laboratories when measuring the frequency of micronuclei and nucleoplasmic bridges in binucleated cells as well as the nuclear division index. The results of the latter study are described in an accompanying paper. It is expected that these scoring criteria will assist in the development of a procedure for calibrating scorers and laboratories so that results from different laboratories for the cytokinesis-block micronucleus assay may be more comparable in the future.",
author = "Michael Fenech and Chang, {W. P.} and M. Kirsch-Volders and N. Holland and S. Bonassi and E. Zeiger",
year = "2003",
month = "1",
day = "10",
doi = "10.1016/S1383-5718(02)00249-8",
language = "English",
volume = "534",
pages = "65--75",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
issn = "1383-5718",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - HUMN project

T2 - Detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures

AU - Fenech, Michael

AU - Chang, W. P.

AU - Kirsch-Volders, M.

AU - Holland, N.

AU - Bonassi, S.

AU - Zeiger, E.

PY - 2003/1/10

Y1 - 2003/1/10

N2 - Criteria for scoring micronuclei and nucleoplasmic bridges in binucleated cells in the cytokinesis-block micronucleus assay for isolated human lymphocyte cultures are described in detail. Morphological characteristics of mononucleated cells, binucleated cells, and multinucleated cells as well as necrotic and apoptotic cells and nuclear buds are also described. These criteria are illustrated by a series of schematic diagrams as well as a comprehensive set of colour photographs that are of practical assistance during the scoring of slides. These scoring criteria, diagrams and photographs have been used in a HUman MicronNucleus (HUMN) project inter-laboratory slide-scoring exercise to evaluate the extent of variability that can be attributable to individual scorers and individual laboratories when measuring the frequency of micronuclei and nucleoplasmic bridges in binucleated cells as well as the nuclear division index. The results of the latter study are described in an accompanying paper. It is expected that these scoring criteria will assist in the development of a procedure for calibrating scorers and laboratories so that results from different laboratories for the cytokinesis-block micronucleus assay may be more comparable in the future.

AB - Criteria for scoring micronuclei and nucleoplasmic bridges in binucleated cells in the cytokinesis-block micronucleus assay for isolated human lymphocyte cultures are described in detail. Morphological characteristics of mononucleated cells, binucleated cells, and multinucleated cells as well as necrotic and apoptotic cells and nuclear buds are also described. These criteria are illustrated by a series of schematic diagrams as well as a comprehensive set of colour photographs that are of practical assistance during the scoring of slides. These scoring criteria, diagrams and photographs have been used in a HUman MicronNucleus (HUMN) project inter-laboratory slide-scoring exercise to evaluate the extent of variability that can be attributable to individual scorers and individual laboratories when measuring the frequency of micronuclei and nucleoplasmic bridges in binucleated cells as well as the nuclear division index. The results of the latter study are described in an accompanying paper. It is expected that these scoring criteria will assist in the development of a procedure for calibrating scorers and laboratories so that results from different laboratories for the cytokinesis-block micronucleus assay may be more comparable in the future.

UR - http://www.scopus.com/inward/record.url?scp=0037427878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037427878&partnerID=8YFLogxK

U2 - 10.1016/S1383-5718(02)00249-8

DO - 10.1016/S1383-5718(02)00249-8

M3 - Article

C2 - 12504755

AN - SCOPUS:0037427878

VL - 534

SP - 65

EP - 75

JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

SN - 1383-5718

IS - 1-2

ER -