Humanized bispecific antibody (mPEG × HER2) rapidly confers PEGylated nanoparticles tumor specificity for multimodality imaging in breast cancer

Yi An Cheng, Tung Ho Wu, Yun Ming Wang, Tian Lu Cheng, I. Ju Chen, Yun Chi Lu, Kuo Hsiang Chuang, Chih Kuang Wang, Chiao Yun Chen, Rui An Lin, Huei Jen Chen, Tzu Yi Liao, En Shuo Liu, Fang Ming Chen

Research output: Contribution to journalArticle

Abstract

Background: Developing a universal strategy to improve the specificity and sensitivity of PEGylated nanoaparticles (PEG-NPs) for assisting in the diagnosis of tumors is important in multimodality imaging. Here, we developed the anti-methoxypolyethylene glycol (mPEG) bispecific antibody (BsAb; mPEG × HER2), which has dual specificity for mPEG and human epidermal growth factor receptor 2 (HER2), with a diverse array of PEG-NPs to confer nanoparticles with HER2 specificity and stronger intensity. Result: We used a one-step formulation to rapidly modify the nanoprobes with mPEG × HER2 and optimized the modified ratio of BsAbs on several PEG-NPs (Lipo-DiR, SPIO, Qdot and AuNP). The αHER2/PEG-NPs could specifically target MCF7/HER2 cells (HER2++) but not MCF7/neo1 cells (HER2+/-). The αHER2/Lipo-DiR and αHER2/SPIO could enhance the sensitivity of untargeted PEG-NPs on MCF7/HER2 (HER2++). In in vivo imaging, αHER2/Lipo-DiR and αHER2/SPIO increased the specific targeting and enhanced PEG-NPs accumulation at 175% and 187% on 24 h, respectively, in HER2-overexpressing tumors. Conclusion: mPEG × HER2, therefore, provided a simple one-step formulation to confer HER2-specific targeting and enhanced sensitivity and contrast intensity on HER2 positive tumors for multimodality imaging. [Figure not available: see fulltext.]

Original languageEnglish
Article number118
JournalJournal of Nanobiotechnology
Volume18
Issue number1
DOIs
Publication statusPublished - Aug 27 2020

Keywords

  • Anti-PEG antibody
  • Bispecific antibody
  • Cancer image
  • Contrast agent
  • Multimodality image
  • One-step formulation
  • PEGylated nanoparticle
  • Polyethylene glycol
  • Tumor specificity

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Humanized bispecific antibody (mPEG × HER2) rapidly confers PEGylated nanoparticles tumor specificity for multimodality imaging in breast cancer'. Together they form a unique fingerprint.

  • Cite this

    Cheng, Y. A., Wu, T. H., Wang, Y. M., Cheng, T. L., Chen, I. J., Lu, Y. C., Chuang, K. H., Wang, C. K., Chen, C. Y., Lin, R. A., Chen, H. J., Liao, T. Y., Liu, E. S., & Chen, F. M. (2020). Humanized bispecific antibody (mPEG × HER2) rapidly confers PEGylated nanoparticles tumor specificity for multimodality imaging in breast cancer. Journal of Nanobiotechnology, 18(1), [118]. https://doi.org/10.1186/s12951-020-00680-9