Human RIF1 encodes an anti-apoptotic factor required for DNA repair

Haibo Wang, Ailian Zhao, Lin Chen, Xueyan Zhong, Ji Liao, Min Gao, Minghua Cai, Dong Hyun Lee, Jing Li, Dipanjan Chowdhury, Yun Gui Yang, Gerd P. Pfeifer, Yun Yen, Xingzhi Xu

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37 Citations (Scopus)

Abstract

Human Rap1-interacting protein 1 (RIF1) contributes to the ataxia telangiectasia, mutated-mediated DNA damage response against the dexterous effect of DNA lesions and plays a critical role in the S-phase checkpoint. However, the molecular mechanisms by which human RIF1 conquers DNA aberrations remain largely unknown. We here showed that inhibition of RIF1 expression by small interfering RNA led to defective homologous recombination-mediated DNA double-strand break repair and sensitized cancer cells to camptothecin or staurosporine treatment. RIF1 underwent caspase-dependent cleavage upon apoptosis. We further found that RIF1 was highly expressed in human breast tumors, and its expression status was positively correlated with differentiation degrees of invasive ductal carcinoma of the breast. Our results suggest that RIF1 encodes an anti-apoptotic factor required for DNA repair and is a potential target for cancer treatment.

Original languageEnglish
Pages (from-to)1314-1319
Number of pages6
JournalCarcinogenesis
Volume30
Issue number8
DOIs
Publication statusPublished - Oct 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research

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    Wang, H., Zhao, A., Chen, L., Zhong, X., Liao, J., Gao, M., Cai, M., Lee, D. H., Li, J., Chowdhury, D., Yang, Y. G., Pfeifer, G. P., Yen, Y., & Xu, X. (2009). Human RIF1 encodes an anti-apoptotic factor required for DNA repair. Carcinogenesis, 30(8), 1314-1319. https://doi.org/10.1093/carcin/bgp136