Human RIF1 encodes an anti-apoptotic factor required for DNA repair

Haibo Wang, Ailian Zhao, Lin Chen, Xueyan Zhong, Ji Liao, Min Gao, Minghua Cai, Dong Hyun Lee, Jing Li, Dipanjan Chowdhury, Yun Gui Yang, Gerd P. Pfeifer, Yun Yen, Xingzhi Xu

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Human Rap1-interacting protein 1 (RIF1) contributes to the ataxia telangiectasia, mutated-mediated DNA damage response against the dexterous effect of DNA lesions and plays a critical role in the S-phase checkpoint. However, the molecular mechanisms by which human RIF1 conquers DNA aberrations remain largely unknown. We here showed that inhibition of RIF1 expression by small interfering RNA led to defective homologous recombination-mediated DNA double-strand break repair and sensitized cancer cells to camptothecin or staurosporine treatment. RIF1 underwent caspase-dependent cleavage upon apoptosis. We further found that RIF1 was highly expressed in human breast tumors, and its expression status was positively correlated with differentiation degrees of invasive ductal carcinoma of the breast. Our results suggest that RIF1 encodes an anti-apoptotic factor required for DNA repair and is a potential target for cancer treatment.

Original languageEnglish
Pages (from-to)1314-1319
Number of pages6
JournalCarcinogenesis
Volume30
Issue number8
DOIs
Publication statusPublished - Oct 1 2009
Externally publishedYes

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DNA Repair
S Phase Cell Cycle Checkpoints
Carcinoma, Ductal, Breast
Camptothecin
Ataxia Telangiectasia
Staurosporine
Double-Stranded DNA Breaks
Homologous Recombination
DNA
Caspases
Small Interfering RNA
DNA Damage
Neoplasms
Apoptosis
Breast Neoplasms
Proteins

ASJC Scopus subject areas

  • Cancer Research

Cite this

Wang, H., Zhao, A., Chen, L., Zhong, X., Liao, J., Gao, M., ... Xu, X. (2009). Human RIF1 encodes an anti-apoptotic factor required for DNA repair. Carcinogenesis, 30(8), 1314-1319. https://doi.org/10.1093/carcin/bgp136

Human RIF1 encodes an anti-apoptotic factor required for DNA repair. / Wang, Haibo; Zhao, Ailian; Chen, Lin; Zhong, Xueyan; Liao, Ji; Gao, Min; Cai, Minghua; Lee, Dong Hyun; Li, Jing; Chowdhury, Dipanjan; Yang, Yun Gui; Pfeifer, Gerd P.; Yen, Yun; Xu, Xingzhi.

In: Carcinogenesis, Vol. 30, No. 8, 01.10.2009, p. 1314-1319.

Research output: Contribution to journalArticle

Wang, H, Zhao, A, Chen, L, Zhong, X, Liao, J, Gao, M, Cai, M, Lee, DH, Li, J, Chowdhury, D, Yang, YG, Pfeifer, GP, Yen, Y & Xu, X 2009, 'Human RIF1 encodes an anti-apoptotic factor required for DNA repair', Carcinogenesis, vol. 30, no. 8, pp. 1314-1319. https://doi.org/10.1093/carcin/bgp136
Wang H, Zhao A, Chen L, Zhong X, Liao J, Gao M et al. Human RIF1 encodes an anti-apoptotic factor required for DNA repair. Carcinogenesis. 2009 Oct 1;30(8):1314-1319. https://doi.org/10.1093/carcin/bgp136
Wang, Haibo ; Zhao, Ailian ; Chen, Lin ; Zhong, Xueyan ; Liao, Ji ; Gao, Min ; Cai, Minghua ; Lee, Dong Hyun ; Li, Jing ; Chowdhury, Dipanjan ; Yang, Yun Gui ; Pfeifer, Gerd P. ; Yen, Yun ; Xu, Xingzhi. / Human RIF1 encodes an anti-apoptotic factor required for DNA repair. In: Carcinogenesis. 2009 ; Vol. 30, No. 8. pp. 1314-1319.
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