Human platelet antigen alleles in 998 taiwanese blood donors determined by sequence-specific primer polymerase chain reaction

Shun Chung Pai, Thierry Burnouf, Jen Wei Chen, Liang In Lin

Research output: Contribution to journalArticle

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Abstract

Polymorphism of human platelet antigens (HPAs) leads to alloimmunizations and immune-mediated platelet disorders including fetal-neonatal alloimmune thrombocytopenia (FNAIT), posttransfusion purpura (PTP), and platelet transfusion refractoriness (PTR). HPA typing and knowledge of antigen frequency in a population are important in particular for the provision of HPA-matched blood components for patients with PTR. We have performed allele genotyping for HPA-1 through -6 and -15 among 998 platelet donors from 6 blood centers in Taiwan using sequence-specific primer polymerase chain reaction. The HPA allele frequency was 99.55, and 0.45% for HPA-1a and -1b; 96.49, and 3.51% for HPA-2a and -2b; 55.81, and 44.19% for HPA-3a and -3b; 99.75, and 0.25% for HPA-4a and -4b; 98.50, and 1.50% for HPA-5a and -5b; 97.75 and 2.25% for HPA-6a and -6b; 53.71 and 46.29% for HPA-15a and -15b. HPA-15b and HPA-3a, may be considered the most important, followed by HPA-2, -6, -1, -5, and -4 systems, as a cause of FNAIT, PTP, and PTR based on allele frequency. HPA-4b and HPA-5b role cannot be excluded based on their immunogenicity. A larger-scale study will now be conducted to confirm these hypotheses and to establish an apheresis donor database for the procurement of HPA-matched apheresis platelets for patients with PTR.

Original languageEnglish
Article number973789
JournalBioMed Research International
Volume2013
DOIs
Publication statusPublished - 2013

Fingerprint

Human Platelet Antigens
Polymerase chain reaction
Blood Donors
Blood
Alleles
Platelets
Polymerase Chain Reaction
Platelet Transfusion
Neonatal Alloimmune Thrombocytopenia
Blood Component Removal
Blood Platelets
Gene Frequency
Polymorphism
Taiwan

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Human platelet antigen alleles in 998 taiwanese blood donors determined by sequence-specific primer polymerase chain reaction. / Pai, Shun Chung; Burnouf, Thierry; Chen, Jen Wei; Lin, Liang In.

In: BioMed Research International, Vol. 2013, 973789, 2013.

Research output: Contribution to journalArticle

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