Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR

Ching Yu Lin, Angel Chao, Yuh Cheng Yang, Hung Hsueh Chou, Chih Ming Ho, Ruey Wen Lin, Ting Chang Chang, Jia Yia Chiou, Fang Yu Chao, Kung Liahng Wang, Tsai Yen Chien, Swei Hsueh, Chu Chun Huang, Chien Jen Chen, Chyong Huey Lai

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background: Type-specific persistence of human papillomavirus (HPV) infection can cause invasive cervical cancer. Objectives: To evaluate the efficacy of HPV detection and typing with a general polymerase chain reaction (PCR)-based genotyping array and to compare it with a type-specific PCR assay. Study design: Four hundred and thirty-three cervical samples were tested with a modified MY11/GP6+ PCR-based reverse-blot assay (EasyChip® HPV Blot; King Car, Taiwan [hereafter HPV Blot]) and with 20 genotypes of L1-type-specific PCR (HPV-6, -11, -16, -18, -31, -33, -35, -39, -45, -51, -52, -53, -56, -58, -59, -62, -66, -68, -70, and -71 [CP8061]). Results: The concordance of the two tests in determining HPV positivity was 96.8% (419/433), with a Cohen's κ = 0.93 (95% CI: 0.90-0.97) and McNemar's test of P = 1.0, which indicates excellent agreement. The overall concordance of the two tests in the identification of type-specific HPV was 91.0% (394/433). Sensitivity (90-100%), specificity (99.2-100%), and accuracy (98.6-100%) rates of HPV Blot against the gold standard were satisfactory for HPV-16, -18, -58, -33, -52, -39, -45, -31, -51, -70 while HPV-71 (63.6%) had suboptimal sensitivity. Though the κ values between the two tests for many individual genotypes could not be reliably calculated because of low positivity, the κ values for HPV-16, -52, and -58 were excellent (0.93, 0.96, and 0.95, respectively). Conclusion: The modified MY11/GP6+ PCR-based HPV Blot assay is accurate and sensitive for detection and genotyping of HPV in cervical swab samples.

Original languageEnglish
Pages (from-to)361-367
Number of pages7
JournalJournal of Clinical Virology
Volume42
Issue number4
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Polymerase Chain Reaction
Human papillomavirus 16
Genotype
Human papillomavirus 11
Human papillomavirus 6
Human papillomavirus 18
Forensic Anthropology
Papillomavirus Infections
Taiwan
Uterine Cervical Neoplasms

Keywords

  • Agreement
  • HPV Blot
  • Human papillomavirus
  • Type-specific PCR

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR. / Lin, Ching Yu; Chao, Angel; Yang, Yuh Cheng; Chou, Hung Hsueh; Ho, Chih Ming; Lin, Ruey Wen; Chang, Ting Chang; Chiou, Jia Yia; Chao, Fang Yu; Wang, Kung Liahng; Chien, Tsai Yen; Hsueh, Swei; Huang, Chu Chun; Chen, Chien Jen; Lai, Chyong Huey.

In: Journal of Clinical Virology, Vol. 42, No. 4, 08.2008, p. 361-367.

Research output: Contribution to journalArticle

Lin, CY, Chao, A, Yang, YC, Chou, HH, Ho, CM, Lin, RW, Chang, TC, Chiou, JY, Chao, FY, Wang, KL, Chien, TY, Hsueh, S, Huang, CC, Chen, CJ & Lai, CH 2008, 'Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR', Journal of Clinical Virology, vol. 42, no. 4, pp. 361-367. https://doi.org/10.1016/j.jcv.2008.03.018
Lin, Ching Yu ; Chao, Angel ; Yang, Yuh Cheng ; Chou, Hung Hsueh ; Ho, Chih Ming ; Lin, Ruey Wen ; Chang, Ting Chang ; Chiou, Jia Yia ; Chao, Fang Yu ; Wang, Kung Liahng ; Chien, Tsai Yen ; Hsueh, Swei ; Huang, Chu Chun ; Chen, Chien Jen ; Lai, Chyong Huey. / Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR. In: Journal of Clinical Virology. 2008 ; Vol. 42, No. 4. pp. 361-367.
@article{64e56095be864b7eb71b03681f110186,
title = "Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR",
abstract = "Background: Type-specific persistence of human papillomavirus (HPV) infection can cause invasive cervical cancer. Objectives: To evaluate the efficacy of HPV detection and typing with a general polymerase chain reaction (PCR)-based genotyping array and to compare it with a type-specific PCR assay. Study design: Four hundred and thirty-three cervical samples were tested with a modified MY11/GP6+ PCR-based reverse-blot assay (EasyChip{\circledR} HPV Blot; King Car, Taiwan [hereafter HPV Blot]) and with 20 genotypes of L1-type-specific PCR (HPV-6, -11, -16, -18, -31, -33, -35, -39, -45, -51, -52, -53, -56, -58, -59, -62, -66, -68, -70, and -71 [CP8061]). Results: The concordance of the two tests in determining HPV positivity was 96.8{\%} (419/433), with a Cohen's κ = 0.93 (95{\%} CI: 0.90-0.97) and McNemar's test of P = 1.0, which indicates excellent agreement. The overall concordance of the two tests in the identification of type-specific HPV was 91.0{\%} (394/433). Sensitivity (90-100{\%}), specificity (99.2-100{\%}), and accuracy (98.6-100{\%}) rates of HPV Blot against the gold standard were satisfactory for HPV-16, -18, -58, -33, -52, -39, -45, -31, -51, -70 while HPV-71 (63.6{\%}) had suboptimal sensitivity. Though the κ values between the two tests for many individual genotypes could not be reliably calculated because of low positivity, the κ values for HPV-16, -52, and -58 were excellent (0.93, 0.96, and 0.95, respectively). Conclusion: The modified MY11/GP6+ PCR-based HPV Blot assay is accurate and sensitive for detection and genotyping of HPV in cervical swab samples.",
keywords = "Agreement, HPV Blot, Human papillomavirus, Type-specific PCR",
author = "Lin, {Ching Yu} and Angel Chao and Yang, {Yuh Cheng} and Chou, {Hung Hsueh} and Ho, {Chih Ming} and Lin, {Ruey Wen} and Chang, {Ting Chang} and Chiou, {Jia Yia} and Chao, {Fang Yu} and Wang, {Kung Liahng} and Chien, {Tsai Yen} and Swei Hsueh and Huang, {Chu Chun} and Chen, {Chien Jen} and Lai, {Chyong Huey}",
year = "2008",
month = "8",
doi = "10.1016/j.jcv.2008.03.018",
language = "English",
volume = "42",
pages = "361--367",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR

AU - Lin, Ching Yu

AU - Chao, Angel

AU - Yang, Yuh Cheng

AU - Chou, Hung Hsueh

AU - Ho, Chih Ming

AU - Lin, Ruey Wen

AU - Chang, Ting Chang

AU - Chiou, Jia Yia

AU - Chao, Fang Yu

AU - Wang, Kung Liahng

AU - Chien, Tsai Yen

AU - Hsueh, Swei

AU - Huang, Chu Chun

AU - Chen, Chien Jen

AU - Lai, Chyong Huey

PY - 2008/8

Y1 - 2008/8

N2 - Background: Type-specific persistence of human papillomavirus (HPV) infection can cause invasive cervical cancer. Objectives: To evaluate the efficacy of HPV detection and typing with a general polymerase chain reaction (PCR)-based genotyping array and to compare it with a type-specific PCR assay. Study design: Four hundred and thirty-three cervical samples were tested with a modified MY11/GP6+ PCR-based reverse-blot assay (EasyChip® HPV Blot; King Car, Taiwan [hereafter HPV Blot]) and with 20 genotypes of L1-type-specific PCR (HPV-6, -11, -16, -18, -31, -33, -35, -39, -45, -51, -52, -53, -56, -58, -59, -62, -66, -68, -70, and -71 [CP8061]). Results: The concordance of the two tests in determining HPV positivity was 96.8% (419/433), with a Cohen's κ = 0.93 (95% CI: 0.90-0.97) and McNemar's test of P = 1.0, which indicates excellent agreement. The overall concordance of the two tests in the identification of type-specific HPV was 91.0% (394/433). Sensitivity (90-100%), specificity (99.2-100%), and accuracy (98.6-100%) rates of HPV Blot against the gold standard were satisfactory for HPV-16, -18, -58, -33, -52, -39, -45, -31, -51, -70 while HPV-71 (63.6%) had suboptimal sensitivity. Though the κ values between the two tests for many individual genotypes could not be reliably calculated because of low positivity, the κ values for HPV-16, -52, and -58 were excellent (0.93, 0.96, and 0.95, respectively). Conclusion: The modified MY11/GP6+ PCR-based HPV Blot assay is accurate and sensitive for detection and genotyping of HPV in cervical swab samples.

AB - Background: Type-specific persistence of human papillomavirus (HPV) infection can cause invasive cervical cancer. Objectives: To evaluate the efficacy of HPV detection and typing with a general polymerase chain reaction (PCR)-based genotyping array and to compare it with a type-specific PCR assay. Study design: Four hundred and thirty-three cervical samples were tested with a modified MY11/GP6+ PCR-based reverse-blot assay (EasyChip® HPV Blot; King Car, Taiwan [hereafter HPV Blot]) and with 20 genotypes of L1-type-specific PCR (HPV-6, -11, -16, -18, -31, -33, -35, -39, -45, -51, -52, -53, -56, -58, -59, -62, -66, -68, -70, and -71 [CP8061]). Results: The concordance of the two tests in determining HPV positivity was 96.8% (419/433), with a Cohen's κ = 0.93 (95% CI: 0.90-0.97) and McNemar's test of P = 1.0, which indicates excellent agreement. The overall concordance of the two tests in the identification of type-specific HPV was 91.0% (394/433). Sensitivity (90-100%), specificity (99.2-100%), and accuracy (98.6-100%) rates of HPV Blot against the gold standard were satisfactory for HPV-16, -18, -58, -33, -52, -39, -45, -31, -51, -70 while HPV-71 (63.6%) had suboptimal sensitivity. Though the κ values between the two tests for many individual genotypes could not be reliably calculated because of low positivity, the κ values for HPV-16, -52, and -58 were excellent (0.93, 0.96, and 0.95, respectively). Conclusion: The modified MY11/GP6+ PCR-based HPV Blot assay is accurate and sensitive for detection and genotyping of HPV in cervical swab samples.

KW - Agreement

KW - HPV Blot

KW - Human papillomavirus

KW - Type-specific PCR

UR - http://www.scopus.com/inward/record.url?scp=46149088452&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46149088452&partnerID=8YFLogxK

U2 - 10.1016/j.jcv.2008.03.018

DO - 10.1016/j.jcv.2008.03.018

M3 - Article

C2 - 18455959

AN - SCOPUS:46149088452

VL - 42

SP - 361

EP - 367

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

IS - 4

ER -