Human mesenchymal stem cells improve myocardial performance in a splenectomized rat model of chronic myocardial infarction

Jen-Fang Liu, Bao Wei Wang, Huei Fong Hung, Hang Chang, Kou-Gi Shyu

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27 Citations (Scopus)

Abstract

Background/Purpose: Cellular therapy has been applied to animal studies and clinical trials for acute or subacute myocardial infarction. Little is known about the effect of cell therapy on chronic myocardial infarction. The goal of this study was to investigate myocardial performance after human bone marrow-derived mesenchymal stem cell (hMSCs) transplantation in rats with chronic myocardial infarction. Methods: The hMSCs were obtained from adult human bone marrow and expanded in vitro. The purity and characteristics of hMSCs were identified by flow cytometry and immunophenotyping. Splenectomy in male rats was performed to prevent immune reaction. One week after splenectomy, ligation of the left anterior descending coronary artery was performed to induce myocardial infarction. Four weeks after ligation of the coronary artery, culture-expanded hMSCs were injected intramyocardially at the left anterior free wall. Left ventricular function measured by echocardiogiaphy, infarct size and immunohistochemical stain were performed to evaluate the effect of the therapy. Results: The engrafted hMSCs were positive for the cardiac marker troponin T. Infarct size (35.4 ± 3.4% vs. 53.3 ± 3.0%, p

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume107
Issue number2
DOIs
Publication statusPublished - Feb 2008

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Keywords

  • Cellular therapy
  • Mesenchymal stem cells
  • Myocardial infarction
  • Myocardial regeneration

ASJC Scopus subject areas

  • Medicine(all)

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