Human cytomegalovirus preferentially infects the neoplastic epithelium of colorectal cancer: A quantitative and histological analysis

Hsin Pai Chen, Jeng Kai Jiang, Cheng Yu Chen, Teh Ying Chou, Yen Chung Chen, Ya Ting Chang, Shiou Fu Lin, Chia Hao Chan, Chih Yung Yang, Chi Hung Lin, Jen Kou Lin, Wen Long Cho, Yu Jiun Chan

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29 Citations (Scopus)

Abstract

Background: It has long been suggested that human cytomegalovirus (HCMV) might be involved in human oncogenesis. However, whether HCMV was associated with colorectal cancer (CRC) was still controversial. Objective: To clarify whether HCMV specifically infects the tumorous tissue of CRC. Study design: Paired tumor and adjacent non-neoplastic CRC specimens were collected from 163 patients. HCMV DNA was detected and quantified through PCR and quantitative real-time PCR. Virus location was determined by in situ hybridization (ISH) of formalin-fixed paraffin-embedded tissue sections with an HCMV-specific probe. Results: By PCR, HCMV DNA was detected in 42.3% (69/163) of the tumor specimens, while only 5.6%(14/163) samples of adjacent non-neoplastic tissue were positive for HCMV (p< 0.0001). Quantitative real-time PCR in 54 sample pairs revealed significantly higher viral copies in the tumor specimens than the adjacent non-neoplastic tissue specimens (p< 0.001). By ISH, the nucleic acids of HCMV were detected in the cytoplasm of neoplastic epithelium. No hybridization was detected in the inflammatory infiltrates, submucosa, or other stromal tissues. Conclusions: HCMV preferentially infects the tumor epithelium of CRC. How the virus subsists in and interacts with the microenvironment of tumor epithelium of CRC should be studied.

Original languageEnglish
Pages (from-to)240-244
Number of pages5
JournalJournal of Clinical Virology
Volume54
Issue number3
DOIs
Publication statusPublished - Jul 1 2012
Externally publishedYes

Keywords

  • Colorectal cancer
  • Human cytomegalovirus
  • In situ hybridization
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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  • Cite this

    Chen, H. P., Jiang, J. K., Chen, C. Y., Chou, T. Y., Chen, Y. C., Chang, Y. T., Lin, S. F., Chan, C. H., Yang, C. Y., Lin, C. H., Lin, J. K., Cho, W. L., & Chan, Y. J. (2012). Human cytomegalovirus preferentially infects the neoplastic epithelium of colorectal cancer: A quantitative and histological analysis. Journal of Clinical Virology, 54(3), 240-244. https://doi.org/10.1016/j.jcv.2012.04.007