Human chromosomal centromere (AATGG)n sequence forms stable structures with unusual base pairs

T. N. Jaishree, Andrew H.J. Wang

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Nine DNA sequences related to the purine strand of the human centromeric satellite (AATGG)n·(CCAAT)n repeat have been studied by two-dimensional nuclear magnetic resonance spectroscopy. Earlier studies have suggested that the structure of (AATGG)n sequence has an equilibrium between the duplex form and a fold-back form. Structural refinement of d(CAATGG) and its related sequences by an NOE-constrained simulated annealing procedure reveals that the duplex form incorporates dynamic type-I G-A base pairs. 1D exchangeable proton NMR data support this model. The reverse sequence motif (GGTAA) destabilizes the structure.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalFEBS Letters
Volume347
Issue number1
DOIs
Publication statusPublished - Jun 20 1994
Externally publishedYes

Keywords

  • Centromere sequence
  • DNA structure
  • NMR
  • Unusual base pairs

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint

Dive into the research topics of 'Human chromosomal centromere (AATGG)n sequence forms stable structures with unusual base pairs'. Together they form a unique fingerprint.

Cite this