Abstract
The nucleotide sequence of the 3' two-thirds of a highly conserved, molecularly cloned human cellular src gene (c-src) has been determined. This region of the c-src gene encodes the tyrosine kinase domain of the cellular src protein (pp60(c-src)) and corresponds to exons 6 through 12 of the chicken c-src gene, as well as nucleotides 545 to 1542 of the Rous sarcoma virus src gene (v-src). The human c-src sequence is very strongly conserved with respect to both the chicken c-src and the Rous sarcoma virus v-src genes, with nearly 90% nucleotide homology observed in this region. Amino acid sequence conservation in this region is even greater; 98% of the amino acids are conserved between human and chicken c-src. Furthermore, the exon sizes and the locations of the exon-intron boundaries are identical in the human and chicken c-src genes. However, sequences within the introns have not been conserved, and the introns within the human c-src gene are significantly larger than the corresponding introns within the chicken c-src gene. The strong amino acid conservation between the carboxy-terminal two-thirds of pp60(c-src) of species as divergent as humans and chickens suggests that this portion of the pp60(c-src) protein specifies one or more functional domains that are of great importance to some aspect of normal cellular growth or differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 1122-1129 |
| Number of pages | 8 |
| Journal | Molecular and Cellular Biology |
| Volume | 5 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jan 1 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
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Human cellular src gene : Nucleotide sequence and derived amino acid sequence of the region coding for the carboxy-terminal two-thirds of pp60(c-src). / Anderson, S. K.; Gibbs, C. P.; Tanaka, A.; Kung, H. J.; Fujita, D. J.
In: Molecular and Cellular Biology, Vol. 5, No. 5, 01.01.1985, p. 1122-1129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human cellular src gene
T2 - Nucleotide sequence and derived amino acid sequence of the region coding for the carboxy-terminal two-thirds of pp60(c-src)
AU - Anderson, S. K.
AU - Gibbs, C. P.
AU - Tanaka, A.
AU - Kung, H. J.
AU - Fujita, D. J.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - The nucleotide sequence of the 3' two-thirds of a highly conserved, molecularly cloned human cellular src gene (c-src) has been determined. This region of the c-src gene encodes the tyrosine kinase domain of the cellular src protein (pp60(c-src)) and corresponds to exons 6 through 12 of the chicken c-src gene, as well as nucleotides 545 to 1542 of the Rous sarcoma virus src gene (v-src). The human c-src sequence is very strongly conserved with respect to both the chicken c-src and the Rous sarcoma virus v-src genes, with nearly 90% nucleotide homology observed in this region. Amino acid sequence conservation in this region is even greater; 98% of the amino acids are conserved between human and chicken c-src. Furthermore, the exon sizes and the locations of the exon-intron boundaries are identical in the human and chicken c-src genes. However, sequences within the introns have not been conserved, and the introns within the human c-src gene are significantly larger than the corresponding introns within the chicken c-src gene. The strong amino acid conservation between the carboxy-terminal two-thirds of pp60(c-src) of species as divergent as humans and chickens suggests that this portion of the pp60(c-src) protein specifies one or more functional domains that are of great importance to some aspect of normal cellular growth or differentiation.
AB - The nucleotide sequence of the 3' two-thirds of a highly conserved, molecularly cloned human cellular src gene (c-src) has been determined. This region of the c-src gene encodes the tyrosine kinase domain of the cellular src protein (pp60(c-src)) and corresponds to exons 6 through 12 of the chicken c-src gene, as well as nucleotides 545 to 1542 of the Rous sarcoma virus src gene (v-src). The human c-src sequence is very strongly conserved with respect to both the chicken c-src and the Rous sarcoma virus v-src genes, with nearly 90% nucleotide homology observed in this region. Amino acid sequence conservation in this region is even greater; 98% of the amino acids are conserved between human and chicken c-src. Furthermore, the exon sizes and the locations of the exon-intron boundaries are identical in the human and chicken c-src genes. However, sequences within the introns have not been conserved, and the introns within the human c-src gene are significantly larger than the corresponding introns within the chicken c-src gene. The strong amino acid conservation between the carboxy-terminal two-thirds of pp60(c-src) of species as divergent as humans and chickens suggests that this portion of the pp60(c-src) protein specifies one or more functional domains that are of great importance to some aspect of normal cellular growth or differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0022390058&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022390058&partnerID=8YFLogxK
U2 - 10.1128/MCB.5.5.1122
DO - 10.1128/MCB.5.5.1122
M3 - Article
AN - SCOPUS:0022390058
VL - 5
SP - 1122
EP - 1129
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 5
ER -