Abstract

We have evaluated the capacity of two human blood fractions to substitute for FBS as growth medium supplement for human and animal cell cultures. Non-anticoagulated blood from volunteer donors (N = 13) was centrifuged to isolate a supernatant serum (SS) and a platelet-rich fibrin (PRF) clot which was squeezed to extract the releasate (PRFR). Both materials were characterized for the content in PDGF-AB, TGF-β1, VEGF, bFGF, EGF, IGF, total protein, albumin, IgG, IgM IgA, fibrinogen, cholesterol, triglycerides, various chemistry analytes and hemoglobin. Cell growth promoting activity of pooled SS and PRFR at 1, 5, and 10% in growth medium was evaluated over 7 days using human (HEK293, MG-63) and animal (SIRC, 3T3) cell lines and two human primary cultures (gingival fibroblasts and periodontal ligaments). Viable cell count was compared to that in cultures in FBS free-medium and 10% FBS supplement. SS and PRFR at 1-10% stimulated cell growth significantly more than FBS-free medium and in a way similar to 10% FBS in all cultures apart from 3T3. These two human blood-derived fibrin releasates are equally efficient to substitute for FBS as supplement for cell cultures and could be useful for specialized applications in regenerative medicine, dentistry and oral implantology, or cell therapy.

Original languageEnglish
Pages (from-to)21-30
Number of pages10
JournalBiologicals
Volume40
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Fibrin
Cell culture
Blood
Cells
Cell growth
Cell Line
Animal cell culture
Chemical analysis
Dentistry
Hemoglobin
Cholesterol
Ligaments
Fibroblasts
Platelets
Epidermal Growth Factor
Fibrinogen
Growth
Immunoglobulin A
Cell Culture Techniques
Vascular Endothelial Growth Factor A

Keywords

  • Blood
  • Cell culture
  • FBS
  • Growth factors
  • Platelet

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Immunology and Microbiology(all)
  • Pharmacology

Cite this

Human blood-derived fibrin releasates : Composition and use for the culture of cell lines and human primary cells. / Burnouf, Thierry; Lee, Chang Yu; Luo, Chin Wan; Kuo, Ya Po; Chou, Ming Li; Wu, Yu Wen; Tseng, Yu Hong; Su, Chen Yao.

In: Biologicals, Vol. 40, No. 1, 01.2012, p. 21-30.

Research output: Contribution to journalArticle

Burnouf, Thierry ; Lee, Chang Yu ; Luo, Chin Wan ; Kuo, Ya Po ; Chou, Ming Li ; Wu, Yu Wen ; Tseng, Yu Hong ; Su, Chen Yao. / Human blood-derived fibrin releasates : Composition and use for the culture of cell lines and human primary cells. In: Biologicals. 2012 ; Vol. 40, No. 1. pp. 21-30.
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