Human adipose-derived stem cell secreted extracellular matrix incorporated into electrospun poly(lactic-co-glycolic acid) nanofibrous dressing for enhancing wound healing

Kao Chun Tang, Kai Chiang Yang, Che Wei Lin, Yi Kai Chen, Ting Yu Lu, Hsien Yeh Chen, Nai Chen Cheng, Jiashing Yu

Research output: Contribution to journalArticle

Abstract

Wound dressing, which prevents dehydration and provides a physical barrier against infection to wound beds, can improve wound healing. The interactions between extracellular matrix (ECM) and growth factors is critical to the healing process. Electrospun nanofibers are promising templates for wound dressings due to the structure similarity to ECM of skin. Otherwise, the ECM secreted by human adipose-derived stem cells (hASCs) is rich in growth factors known to enhance wound healing. Accordingly, we propose that the PLGA nanofibrous template incorporated with hASCs-secreted ECM may enhance wound healing. In this study, PLGA nanofibrous matrixes with an aligned or a random structure were prepared by electrospinning. Human ASCs cultured on the aligned matrix had a better viability and produced a larger amount of ECM relative to that of random one. After 7 days' cultivation, the hASCs on aligned PLGA substrates underwent decellularization to fabricate cECM/PLGA dressings. By using immunohistochemical staining against F-actin and cell nucleus, the removal of cellular components was verified. However, the type I collagen and laminin were well preserved on the cECM/PLGA nanofibrous matrixes. In addition, this substrate was hydrophilic, with appropriate mechanical strength to act as a wound dressing. The L929 fibroblasts had good activity, survival and proliferation on the cECM/PLGA meshes. In addition, the cECM/PLGA nanofibrous dressings improved the wound healing of surgically created full-thickness skin excision in a mouse model. This hASCs-secreted ECM incorporated into electrospun PLGA nanofibrous could be a promising dressing for enhancing wound healing.

Original languageEnglish
Article number1609
JournalPolymers
Volume11
Issue number10
DOIs
Publication statusPublished - Oct 1 2019

Fingerprint

Stem cells
Acids
Skin
Electrospinning
Substrates
Fibroblasts
Nanofibers
Dehydration
Collagen
Strength of materials
Intercellular Signaling Peptides and Proteins
Cells
Milk
polylactic acid-polyglycolic acid copolymer
Laminin
Collagen Type I
Actins

Keywords

  • Electrospinning
  • Extracellular matrix
  • Human adipose-derived stem cells
  • Wound healing

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

Cite this

Human adipose-derived stem cell secreted extracellular matrix incorporated into electrospun poly(lactic-co-glycolic acid) nanofibrous dressing for enhancing wound healing. / Tang, Kao Chun; Yang, Kai Chiang; Lin, Che Wei; Chen, Yi Kai; Lu, Ting Yu; Chen, Hsien Yeh; Cheng, Nai Chen; Yu, Jiashing.

In: Polymers, Vol. 11, No. 10, 1609, 01.10.2019.

Research output: Contribution to journalArticle

Tang, Kao Chun ; Yang, Kai Chiang ; Lin, Che Wei ; Chen, Yi Kai ; Lu, Ting Yu ; Chen, Hsien Yeh ; Cheng, Nai Chen ; Yu, Jiashing. / Human adipose-derived stem cell secreted extracellular matrix incorporated into electrospun poly(lactic-co-glycolic acid) nanofibrous dressing for enhancing wound healing. In: Polymers. 2019 ; Vol. 11, No. 10.
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AU - Lin, Che Wei

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AU - Lu, Ting Yu

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AU - Cheng, Nai Chen

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AB - Wound dressing, which prevents dehydration and provides a physical barrier against infection to wound beds, can improve wound healing. The interactions between extracellular matrix (ECM) and growth factors is critical to the healing process. Electrospun nanofibers are promising templates for wound dressings due to the structure similarity to ECM of skin. Otherwise, the ECM secreted by human adipose-derived stem cells (hASCs) is rich in growth factors known to enhance wound healing. Accordingly, we propose that the PLGA nanofibrous template incorporated with hASCs-secreted ECM may enhance wound healing. In this study, PLGA nanofibrous matrixes with an aligned or a random structure were prepared by electrospinning. Human ASCs cultured on the aligned matrix had a better viability and produced a larger amount of ECM relative to that of random one. After 7 days' cultivation, the hASCs on aligned PLGA substrates underwent decellularization to fabricate cECM/PLGA dressings. By using immunohistochemical staining against F-actin and cell nucleus, the removal of cellular components was verified. However, the type I collagen and laminin were well preserved on the cECM/PLGA nanofibrous matrixes. In addition, this substrate was hydrophilic, with appropriate mechanical strength to act as a wound dressing. The L929 fibroblasts had good activity, survival and proliferation on the cECM/PLGA meshes. In addition, the cECM/PLGA nanofibrous dressings improved the wound healing of surgically created full-thickness skin excision in a mouse model. This hASCs-secreted ECM incorporated into electrospun PLGA nanofibrous could be a promising dressing for enhancing wound healing.

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