Human acellular cartilage matrix powders as a biological scaffold for cartilage tissue engineering with synovium-derived mesenchymal stem cells

Chih Hung Chang, Chia Chun Chen, Cheng Hao Liao, Feng Huei Lin, Yuan Ming Hsu, Hsu Wei Fang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In our previous study, we found that cartilage fragments from osteoarthritic knee promoted chondrogenesis of mesenchymal stem cells. In this study, we further transformed the cartilage tissues into acellular cartilage matrix (ACM) and explored the feasibility of using ACM as a biological scaffold. Nonworn parts of cartilage tissues were obtained during total knee arthroplasty (TKA) surgery and were successfully fabricated into ACM powders. The ACM powders and human synovium-derived mesenchymal stem cells (SMSCs) were mixed into collagen gel for in vitro culture. Histological results showed a synergistic effect of ACM powders and chondrogenic growth factors in the formation of engineered cartilage. The findings of real-time polymerase chain reaction (PCR) suggested that ACM powders had the potential of promoting type II collagen gene expression in the growth factors-absent environment. Moreover, with growth factors induction, the ACM powders could reduce the hypertrophy in chondrogenesis of SMSCs. In summary, ACM powders could serve as a functional scaffold that benefited the chondrogenesis of SMSCs for cartilage tissue engineering.

Original languageEnglish
Pages (from-to)2248-2257
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume102
Issue number7
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

Cartilage
Scaffolds (biology)
Stem cells
Tissue engineering
Powders
Intercellular Signaling Peptides and Proteins
Collagen
Scaffolds
Tissue
Arthroplasty
Collagen Type II
Polymerase chain reaction
Gene expression
Surgery
Gels

Keywords

  • acellular cartilage matrix (ACM)
  • chondrogenesis
  • synovium-derived mesenchymal stem cells (SMSCs)
  • tissue engineering

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Human acellular cartilage matrix powders as a biological scaffold for cartilage tissue engineering with synovium-derived mesenchymal stem cells. / Chang, Chih Hung; Chen, Chia Chun; Liao, Cheng Hao; Lin, Feng Huei; Hsu, Yuan Ming; Fang, Hsu Wei.

In: Journal of Biomedical Materials Research - Part A, Vol. 102, No. 7, 01.01.2014, p. 2248-2257.

Research output: Contribution to journalArticle

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