How yeast can be used as a genetic platform to explore virus-host interactions: From 'omics' to functional studies

Peter D. Nagy, Judit Pogany, Jing Yi Lin

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The yeast Saccharomyces cerevisiae is an advanced model organism that has emerged as an effective host to gain insights into the intricate interactions of viruses with host cells. RNA viruses have limited coding potential and need to coopt numerous host cellular factors to facilitate their replication. To identify the host factors subverted by viruses, high-throughput genomics and global proteomics approaches have been performed with plant viruses such as brome mosaic virus (BMV) and tomato bushy stunt virus (TBSV). Accordingly, several hundred susceptibility and restriction factors for BMV and TBSV have been identified using yeast as a model host. Amazingly, host factors affecting viral genetic recombination and evolution have also been identified in genome-wide screens in yeast. The roles of many yeast host factors involved in various steps of the viral replication process have been validated by exploiting the orthologous genes in plant hosts. This Opinion summarizes the advantages of using simple viruses and yeast model host to advance our general understanding of virus-host interactions. The knowledge gained on host factors could lead to novel specific or broad-range resistance and antiviral tools against viruses.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalTrends in Microbiology
Volume22
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Yeasts
Viruses
Tombusvirus
Bromovirus
Plant Viruses
Plant Genes
Molecular Evolution
RNA Viruses
Genomics
Proteomics
Genetic Recombination
Antiviral Agents
Saccharomyces cerevisiae
Genome

Keywords

  • Genome-wide screens
  • Host factors
  • Protein-protein interaction
  • RNA-protein interaction
  • Viral replicase complex
  • Virus replication
  • Yeast as a host

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)
  • Microbiology
  • Virology
  • Medicine(all)

Cite this

How yeast can be used as a genetic platform to explore virus-host interactions : From 'omics' to functional studies. / Nagy, Peter D.; Pogany, Judit; Lin, Jing Yi.

In: Trends in Microbiology, Vol. 22, No. 6, 2014, p. 309-316.

Research output: Contribution to journalArticle

Nagy, Peter D. ; Pogany, Judit ; Lin, Jing Yi. / How yeast can be used as a genetic platform to explore virus-host interactions : From 'omics' to functional studies. In: Trends in Microbiology. 2014 ; Vol. 22, No. 6. pp. 309-316.
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